| |  Online-Ressource |
|---|
| Verfasst von: | Weißenbruch, Kai [VerfasserIn]  |
|---|
| | Grewe, Justin [VerfasserIn] |
|---|
| | Hippler, Marc [VerfasserIn] |
|---|
| | Fladung, Magdalena [VerfasserIn] |
|---|
| | Tremmel, Moritz [VerfasserIn] |
|---|
| | Stricker, Kathrin [VerfasserIn] |
|---|
| | Schwarz, Ulrich S. [VerfasserIn] |
|---|
| | Bastmeyer, Martin [VerfasserIn] |
|---|
| Titel: | Distinct roles of nonmuscle myosin II isoforms for establishing tension and elasticity during cell morphodynamics |
|---|
| Verf.angabe: | Kai Weißenbruch, Justin Grewe, Marc Hippler, Magdalena Fladung, Moritz Tremmel, Kathrin Stricker, Ulrich Sebastian Schwarz, Martin Bastmeyer |
|---|
| E-Jahr: | 2021 |
|---|
| Jahr: | 10 August 2021 |
|---|
| Umfang: | 35 S. |
|---|
| Teil: | volume:10 |
|---|
| | year:2021 |
|---|
| | elocationid:e71888 |
|---|
| | pages:1-35 |
|---|
| | extent:35 |
|---|
| Fussnoten: | Gesehen am 07.10.2021 |
|---|
| Titel Quelle: | Enthalten in: eLife |
|---|
| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
|---|
| Jahr Quelle: | 2021 |
|---|
| Band/Heft Quelle: | 10(2021), Artikel-ID e71888, Seite 1-35 |
|---|
| ISSN Quelle: | 2050-084X |
|---|
| Abstract: | Nonmuscle myosin II (NM II) is an integral part of essential cellular processes, including adhesion and migration. Mammalian cells express up to three isoforms termed NM IIA, B, and C. We used U2OS cells to create CRISPR/Cas9-based knockouts of all three isoforms and analyzed the phenotypes on homogenously coated surfaces, in collagen gels, and on micropatterned substrates. In contrast to homogenously coated surfaces, a structured environment supports a cellular phenotype with invaginated actin arcs even in the absence of NM IIA-induced contractility. A quantitative shape analysis of cells on micropatterns combined with a scale-bridging mathematical model reveals that NM IIA is essential to build up cellular tension during initial stages of force generation, while NM IIB is necessary to elastically stabilize NM IIA-generated tension. A dynamic cell stretch/release experiment in a three-dimensional scaffold confirms these conclusions and in addition reveals a novel role for NM IIC, namely the ability to establish tensional homeostasis. |
|---|
| DOI: | doi:10.7554/eLife.71888 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.7554/eLife.71888 |
|---|
| | Volltext: https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=DOISource&SrcApp=WOS&KeyAID=10.7554%2Felife.7 .... |
|---|
| | DOI: https://doi.org/10.7554/eLife.71888 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | ablation |
|---|
| | actin |
|---|
| | adhesion |
|---|
| | contractile |
|---|
| | dynamics |
|---|
| | extracellular-matrix geometry |
|---|
| | force |
|---|
| | generation |
|---|
| | self-assembled monolayers |
|---|
| | stress fibers |
|---|
| K10plus-PPN: | 1772761680 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Distinct roles of nonmuscle myosin II isoforms for establishing tension and elasticity during cell morphodynamics / Weißenbruch, Kai [VerfasserIn]; 10 August 2021 (Online-Ressource)
