| |  Online-Ressource |
|---|
| Verfasst von: | Samra, Vanessa [VerfasserIn]  |
|---|
| | Drews, Oliver [VerfasserIn] |
|---|
| | Costina, Victor [VerfasserIn] |
|---|
| | Bierbaum, Miriam [VerfasserIn] |
|---|
| | Jawhar, Ahmed [VerfasserIn] |
|---|
| | Röhl, Henning [VerfasserIn] |
|---|
| | Weiß, Christel [VerfasserIn] |
|---|
| | Brendel, Susanne [VerfasserIn] |
|---|
| | Kleiner, Helga [VerfasserIn] |
|---|
| | Flach, Johanna [VerfasserIn] |
|---|
| | Spiess, Birgit [VerfasserIn] |
|---|
| | Seifarth, Wolfgang [VerfasserIn] |
|---|
| | Nowak, Daniel [VerfasserIn] |
|---|
| | Hofmann, Wolf-Karsten [VerfasserIn] |
|---|
| | Fabarius, Alice [VerfasserIn] |
|---|
| | Popp, Henning [VerfasserIn] |
|---|
| Titel: | Proteins marking the sequence of genotoxic signaling from irradiated mesenchymal stromal cells to CD34+ cells |
|---|
| Verf.angabe: | Vanessa Kohl, Oliver Drews, Victor Costina, Miriam Bierbaum, Ahmed Jawhar, Henning Roehl, Christel Weiss, Susanne Brendel, Helga Kleiner, Johanna Flach, Birgit Spiess, Wolfgang Seifarth, Daniel Nowak, Wolf-Karsten Hofmann, Alice Fabarius and Henning D. Popp |
|---|
| E-Jahr: | 2021 |
|---|
| Jahr: | 29 May 2021 |
|---|
| Umfang: | 21 S. |
|---|
| Fussnoten: | Gesehen am 11.10.2021 |
|---|
| Titel Quelle: | Enthalten in: International journal of molecular sciences |
|---|
| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
|---|
| Jahr Quelle: | 2021 |
|---|
| Band/Heft Quelle: | 22(2021), 11, Artikel-ID 5844, Seite 1-21 |
|---|
| ISSN Quelle: | 1422-0067 |
|---|
| | 1661-6596 |
|---|
| Abstract: | Non-targeted effects (NTE) of ionizing radiation may initiate myeloid neoplasms (MN). Here, protein mediators (I) in irradiated human mesenchymal stromal cells (MSC) as the NTE source, (II) in MSC conditioned supernatant and (III) in human bone marrow CD34+ cells undergoing genotoxic NTE were investigated. Healthy sublethal irradiated MSC showed significantly increased levels of reactive oxygen species. These cells responded by increasing intracellular abundance of proteins involved in proteasomal degradation, protein translation, cytoskeleton dynamics, nucleocytoplasmic shuttling, and those with antioxidant activity. Among the increased proteins were THY1 and GNA11/14, which are signaling proteins with hitherto unknown functions in the radiation response and NTE. In the corresponding MSC conditioned medium, the three chaperones GRP78, CALR, and PDIA3 were increased. Together with GPI, these were the only four altered proteins, which were associated with the observed genotoxic NTE. Healthy CD34+ cells cultured in MSC conditioned medium suffered from more than a six-fold increase in γH2AX focal staining, indicative for DNA double-strand breaks, as well as numerical and structural chromosomal aberrations within three days. At this stage, five proteins were altered, among them IQGAP1, HMGB1, and PA2G4, which are involved in malign development. In summary, our data provide novel insights into three sequential steps of genotoxic signaling from irradiated MSC to CD34+ cells, implicating that induced NTE might initiate the development of MN. |
|---|
| DOI: | doi:10.3390/ijms22115844 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/ijms22115844 |
|---|
| | Volltext: https://www.mdpi.com/1422-0067/22/11/5844 |
|---|
| | DOI: https://doi.org/10.3390/ijms22115844 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | CD34+ cells |
|---|
| | genotoxic signals |
|---|
| | irradiation |
|---|
| | mesenchymal stromal cells |
|---|
| | myeloid neoplasms |
|---|
| | non-targeted effects |
|---|
| K10plus-PPN: | 1773261339 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Proteins marking the sequence of genotoxic signaling from irradiated mesenchymal stromal cells to CD34+ cells / Samra, Vanessa [VerfasserIn]; 29 May 2021 (Online-Ressource)
