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| Verfasst von: | Kallifatidis, Georgios [VerfasserIn]  |
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| | Beckermann, Benjamin Michael [VerfasserIn] |
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| | Groth, Ariane [VerfasserIn] |
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| | Schubert, Mario [VerfasserIn] |
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| | Apel, Anja [VerfasserIn] |
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| | Khamidjanov, Akmal [VerfasserIn] |
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| | Ryschich, Eduard [VerfasserIn] |
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| | Wenger, Till [VerfasserIn] |
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| | Wagner, Wolfgang [VerfasserIn] |
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| | Diehlmann, Anke [VerfasserIn] |
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| | Saffrich, Rainer [VerfasserIn] |
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| | Krause, Ulf [VerfasserIn] |
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| | Eckstein, Volker [VerfasserIn] |
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| | Mattern, Jürgen [VerfasserIn] |
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| | Chai, Minqiang [VerfasserIn] |
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| | Schütz, Günther [VerfasserIn] |
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| | Ho, Anthony Dick [VerfasserIn] |
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| | Gebhard, Martha-Maria [VerfasserIn] |
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| | Büchler, Markus W. [VerfasserIn] |
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| | Friess, Helmut [VerfasserIn] |
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| | Büchler, Peter [VerfasserIn] |
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| | Herr, Ingrid [VerfasserIn] |
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| Titel: | Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer |
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| Verf.angabe: | G. Kallifatidis, B.M. Beckermann, A. Groth, M. Schubert, A. Apel, A. Khamidjanov, E. Ryschich, T. Wenger, W. Wagner, A. Diehlmann, R. Saffrich, U. Krause, V. Eckstein, J. Mattern, M. Chai, G. Schütz, A.D. Ho, M.M. Gebhard, M.W. Büchler, H. Friess, P. Büchler and I. Herr |
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| E-Jahr: | 2008 |
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| Jahr: | 18 January 2008 |
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| Umfang: | 10 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 14.10.2021 |
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| Titel Quelle: | Enthalten in: Cancer gene therapy |
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| Ort Quelle: | New York, NY : Nature Publ. Group, 1999 |
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| Jahr Quelle: | 2008 |
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| Band/Heft Quelle: | 15(2008), 4, Seite 231-240 |
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| ISSN Quelle: | 1476-5500 |
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| Abstract: | Genetic modification of human bone marrow mesenchymal stem cells (MSC) is highly valuable for their exploitation in basic science and therapeutic applications, for example in cancer. We present here a new, fast and easy-to-use method to enrich a functional population of lentiviral (LV)-transduced MSC expressing enhanced green fluorescent protein (eGFP). We replaced the eGFP gene by a fusion gene of puromycin acetyltransferase and eGFP. Upon LV gene transfer and puromycin selection, we quickly obtained a pure transduced MSC population, in which growth, differentiation capacity and migration preferences were not compromised. Furthermore, we are the first to report the migration velocity of MSC among which 30% were moving and velocity of about 15 μm h−1 was not altered by LV transduction. Manipulated MSC underwent senescence one passage earlier than non-transduced cells, suggesting the use for therapeutic intervention in early passage numbers. Upon tail vein application in nude mice, the majority of LV-transduced MSC could be detected in human orthotopic pancreatic tumor xenografts and to a minor extent in mouse liver, kidney and lung. Together, LV transduction of genes to MSC followed by puromycin selection is a powerful tool for basic research and improves the therapeutic prospects of MSC as vehicles in gene therapy. |
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| DOI: | doi:10.1038/sj.cgt.7701097 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/sj.cgt.7701097 |
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| | Volltext: https://www.nature.com/articles/7701097 |
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| | DOI: https://doi.org/10.1038/sj.cgt.7701097 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1773861697 |
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| Verknüpfungen: | → Zeitschrift |
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Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer / Kallifatidis, Georgios [VerfasserIn]; 18 January 2008 (Online-Ressource)
