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Verfasst von:Löffler, Harald [VerfasserIn]   i
 Bochtler, Tilmann [VerfasserIn]   i
 Tews, Britta [VerfasserIn]   i
 Tews, Björn [VerfasserIn]   i
 Ho, Anthony Dick [VerfasserIn]   i
 Lukas, Jiri [VerfasserIn]   i
 Bartek, Jiri [VerfasserIn]   i
 Krämer, Alwin [VerfasserIn]   i
Titel:DNA damage-induced accumulation of centrosomal Chk1 contributes to its checkpoint function
Verf.angabe:Harald Löffler, Tilmann Bochtler, Britta Fritz, Björn Tews, Anthony D. Ho, Jiri Lukas, Jiri Bartek, Alwin Krämer
E-Jahr:2007
Jahr:19 Oct 2007
Umfang:8 S.
Illustrationen:Illustrationen
Teil:volume:6
 year:2007
 number:20
 pages:2541-2548
 extent:8
Fussnoten:Gesehen am 20.10.2021
Titel Quelle:Enthalten in: Cell cycle
Ort Quelle:Abingdon : Taylor & Francis Group, 2002
Jahr Quelle:2007
Band/Heft Quelle:6(2007), 20, Seite 2541-2548
ISSN Quelle:1551-4005
Abstract:The checkpoint kinase Chk1 is an established transducer of ATR- and ATM-dependent signalling in response to DNA damage. In addition to its nuclear localization, Chk1 localizes to interphase centrosomes and thereby negatively regulates entry into mitosis by preventing premature activation of cyclin B-Cdk1 during unperturbed cell cycles. Here, we demonstrate that DNA damage caused by ultraviolet irradiation or hydroxyurea treatment leads to centrosomal accumulation of endogenous Chk1 in normal human BJ fibroblasts and in ATR- or ATM-deficient fibroblasts. Chemical inhibition of ATR/ATM by caffeine led to enhanced centrosomal Chk1 deposition associated with nuclear Chk1 depletion. In contrast to normal or ATM-deficient fibroblasts, genetically ATR-deficient Seckel-fibroblasts showed detectable constitutive centrosomal accumulation of Chk1 even in the absence of exogenous insults. After DNA damage, the centrosomal fraction of Chk1 was found to be phosphorylated at ATR/ATM phosphorylation sites. Forced immobilization of kinase-inactive but not wild-type Chk1 to centrosomes resulted in a G2/M checkpoint defect. Finally, both DNA damage, and forced centrosomal expression of Chk1 in the absence of genotoxic treatments, induced centrosome amplification in a subset of cells, a phenomenon which could be suppressed by inhibition of ATM/ATR-mediated signaling. Taken together, our results suggest that accumulation of phosphorylated Chk1 at centrosomes constitutes an additional element in the DNA damage response. Centrosomal Chk1 induces G2/M cell cycle arrest and may evoke centrosome amplification, the latter possibly providing a backup mechanism for elimination of cells with impaired DNA damage checkpoints operating earlier during the cell cycle.
DOI:doi:10.4161/cc.6.20.4810
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.4161/cc.6.20.4810
 DOI: https://doi.org/10.4161/cc.6.20.4810
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1774620219
Verknüpfungen:→ Zeitschrift

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