Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Mulindwa, Julius [VerfasserIn]   i
 Ssentamu, Geofrey [VerfasserIn]   i
 Matovu, Enock [VerfasserIn]   i
 Marucha, Kevin [VerfasserIn]   i
 Aresta-Branco, Francisco [VerfasserIn]   i
 Helbig, Claudia [VerfasserIn]   i
 Clayton, Christine [VerfasserIn]   i
Titel:In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes
Verf.angabe:Julius Mulindwa, Geofrey Ssentamu, Enock Matovu, Kevin Kamanyi Marucha, Francisco Aresta-Branco, Claudia Helbig, Christine Clayton
E-Jahr:2021
Jahr:September 13, 2021
Umfang:20 S.
Fussnoten:Gesehen am 04.11.2021
Titel Quelle:Enthalten in: Public Library of SciencePLoS neglected tropical diseases
Ort Quelle:Lawrence, Kan. : PLoS, 2007
Jahr Quelle:2021
Band/Heft Quelle:15(2021) vom: Sept., Artikel-ID e0009738, Seite 1-20
ISSN Quelle:1935-2735
Abstract:Most researchers who study unicellular eukaryotes work with an extremely limited number of laboratory-adapted isolates that were obtained from the field decades ago, but the effects of passage in laboratory rodents, and adaptation to in vitro culture, have been little studied. For example, the vast majority of studies of Trypanosoma brucei biology have concentrated on just two strains, Lister 427 and EATRO1125, which were taken from the field over half a century ago and have since have undergone innumerable passages in rodents and culture. We here describe two new Trypanosoma brucei brucei strains. MAK65 and MAK98, which have undergone only 3 rodent passages since isolation from Ugandan cattle. High-coverage sequencing revealed that adaptation of the parasites to culture was accompanied by changes in gene copy numbers. T. brucei has so far been considered to be uniformly diploid, but we also found trisomy of chromosome 5 not only in one Lister 427 culture, but also in the MAK98 field isolate. Trisomy of chromosome 6, and increased copies of other chromosome segments, were also seen in established cultured lines. The two new T. brucei strains should be useful to researchers interested in trypanosome differentiation and pathogenicity. Initial results suggested that the two strains have differing infection patterns in rodents. MAK65 is uniformly diploid and grew more reproducibly in bloodstream-form culture than MAK98.
DOI:doi:10.1371/journal.pntd.0009738
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.pntd.0009738
 Volltext: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009738
 DOI: https://doi.org/10.1371/journal.pntd.0009738
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Blood
 Genomics
 Mammalian genomics
 Parasitic diseases
 Rodents
 Trypanosoma
 Trypanosoma brucei
 Trypanosoma brucei gambiense
K10plus-PPN:1776195388
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68796851   QR-Code
zum Seitenanfang