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Verfasst von:Fröhlich, Benjamin [VerfasserIn]   i
 Dasanna, Anil K. [VerfasserIn]   i
 Lansche, Christine [VerfasserIn]   i
 Czajor, Julian [VerfasserIn]   i
 Sanchez, Cecilia P. [VerfasserIn]   i
 Cyrklaff, Marek [VerfasserIn]   i
 Yamamoto, Akihisa [VerfasserIn]   i
 Craig, Alister [VerfasserIn]   i
 Schwarz, Ulrich S. [VerfasserIn]   i
 Lanzer, Michael [VerfasserIn]   i
 Tanaka, Motomu [VerfasserIn]   i
Titel:Functionalized supported membranes for quantifying adhesion of P. falciparum-infected erythrocytes
Verf.angabe:Benjamin Fröhlich, Anil K. Dasanna, Christine Lansche, Julian Czajor, Cecilia P. Sanchez, Marek Cyrklaff, Akihisa Yamamoto, Alister Craig, Ulrich S. Schwarz, Michael Lanzer, and Motomu Tanaka
E-Jahr:2021
Jahr:9 July 2021
Umfang:14 S.
Fussnoten:Gesehen am 05.11.2021
Titel Quelle:Enthalten in: Biophysical journal
Ort Quelle:Cambridge, Mass. : Cell Press, 1960
Jahr Quelle:2021
Band/Heft Quelle:120(2021), 16 vom: 17. Aug., Seite 3315-3328
ISSN Quelle:1542-0086
Abstract:The pathology of Plasmodium falciparum malaria is largely defined by the cytoadhesion of infected erythrocytes to the microvascular endothelial lining. The complexity of the endothelial surface and the large range of interactions available for the infected erythrocyte via parasite-encoded adhesins make analysis of critical contributions during cytoadherence challenging to define. Here, we have explored supported membranes functionalized with two important adhesion receptors, ICAM1 or CD36, as a quantitative biomimetic surface to help understand the processes involved in cytoadherence. Parasitized erythrocytes bound to the receptor-functionalized membranes with high efficiency and selectivity under both static and flow conditions, with infected wild-type erythrocytes displaying a higher binding capacity than do parasitized heterozygous sickle cells. We further show that the binding efficiency decreased with increasing intermolecular receptor distance and that the cell-surface contacts were highly dynamic and increased with rising wall shear stress as the cell underwent a shape transition. Computer simulations using a deformable cell model explained the wall-shear-stress-induced dynamic changes in cell shape and contact area via the specific physical properties of erythrocytes, the density of adhesins presenting knobs, and the lateral movement of receptors in the supported membrane.
DOI:doi:10.1016/j.bpj.2021.07.003
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.bpj.2021.07.003
 Volltext: https://www.sciencedirect.com/science/article/pii/S000634952100552X
 DOI: https://doi.org/10.1016/j.bpj.2021.07.003
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1776238907
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