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Verfasst von:Einarsson, Elin [VerfasserIn]   i
 Lassadi, Imen [VerfasserIn]   i
 Zielinski, Jana [VerfasserIn]   i
 Guan, Qingtian [VerfasserIn]   i
 Wyler, Tobias [VerfasserIn]   i
 Pain, Arnab [VerfasserIn]   i
 Gornik, Sebastian G. [VerfasserIn]   i
 Waller, Ross F. [VerfasserIn]   i
Titel:Development of the myzozoan aquatic parasite Perkinsus marinus as a versatile experimental genetic model organism
Verf.angabe:Elin Einarsson, Imen Lassadi, Jana Zielinski, Qingtian Guan, Tobias Wyler, Arnab Pain, Sebastian G. Gornik, and Ross F. Waller
E-Jahr:2021
Jahr:11 August 2021
Umfang:15 S.
Fussnoten:Gesehen am 05.11.2021
Titel Quelle:Enthalten in: Protist
Ort Quelle:München : Elsevier, 1998
Jahr Quelle:2021
Band/Heft Quelle:172(2021), 4 vom: Aug., Artikel-ID 125830, Seite 1-15
ISSN Quelle:1618-0941
Abstract:The phylum Perkinsozoa is an aquatic parasite lineage that has devastating effects on commercial and natural mollusc populations, and also comprises parasites of algae, fish and amphibians. They are related to dinoflagellates and apicomplexans and thus offer excellent genetic models for both parasitological and evolutionary studies. Genetic transformation was previously achieved for Perkinsus spp. but with few tools for transgene expression and limited selection efficacy. We sought to expand the power of experimental genetic tools for Perkinsus using P. marinus as a model. We constructed a modular plasmid assembly system for expression of multiple genes simultaneously. We developed efficient selection systems for three drugs, puromycin, bleomycin and blasticidin, that are effective in as little as three weeks. We developed eleven new promoters of variable expression strength. Furthermore, we identified that genomic integration of transgenes is predominantly via non-homologous recombination but with transgene fragmentation including deletion of some elements. To counter these dynamic processes, we show that bi-cistronic transcripts using the viral 2A peptides can couple selection to the maintenance of the expression of a transgene of interest. Collectively, these new tools and insights provide great new capacity to genetically modify and study Perkinsus as an aquatic parasite and evolutionary model.
DOI:doi:10.1016/j.protis.2021.125830
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.protis.2021.125830
 Volltext: https://www.sciencedirect.com/science/article/pii/S1434461021000390
 DOI: https://doi.org/10.1016/j.protis.2021.125830
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:drug-selection
 myzozoa
 parasite
 transformation
K10plus-PPN:1776256107
Verknüpfungen:→ Zeitschrift

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