Identification of a new HLA-A2-restricted T-cell epitope within HM1.24 as immunotherapy target for multiple myeloma

• Verfasst von: Hundemer, Michael [VerfasserIn]
• Verfasst von: Schmidt, Stefanie [VerfasserIn]
• Verfasst von: Condomines, Maud [VerfasserIn]
• Verfasst von: Lupu, Alaviana [VerfasserIn]
• Verfasst von: Hose, Dirk [VerfasserIn]
• Verfasst von: Moos, Marion [VerfasserIn]
• Verfasst von: Cremer, Friedrich Walter [VerfasserIn]
• Verfasst von: Kleist, Christian [VerfasserIn]
• Verfasst von: Terness, Peter [VerfasserIn]
• Verfasst von: Belle, Sebastian [VerfasserIn]
• Verfasst von: Ho, Anthony Dick [VerfasserIn]
• Verfasst von: Goldschmidt, Hartmut [VerfasserIn]
• Verfasst von: Klein, Bernard [VerfasserIn]
• Verfasst von: Christensen, Olaf [VerfasserIn]
• Titel: Identification of a new HLA-A2-restricted T-cell epitope within HM1.24 as immunotherapy target for multiple myeloma
• Verf.angabe: Michael Hundemer, Stefanie Schmidt, Maud Condomines, Alaviana Lupu, Dirk Hose, Marion Moos, Friedrich Cremer, Christian Kleist, Peter Terness, Sebastian Belle, Anthony D. Ho, Hartmut Goldschmidt, Bernard Klein, and Olaf Christensen
• E-Jahr: 2006
• Jahr: [April 2006]
• Umfang: 11 S.
• Illustrationen: Diagramme
• Fussnoten: Gesehen am 05.11.2021
• Titel Quelle: Enthalten in: Experimental hematology
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1999
• Jahr Quelle: 2006
• Band/Heft Quelle: 34(2006), 4 vom: Apr., Seite 486-496
• ISSN Quelle: 1873-2399
• DOI: doi:10.1016/j.exphem.2006.01.008
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1776280016

Abstract

Objective - The aim of this study was identification of human leukocyte antigen (HLA)-A2-restricted T-cell epitopes within the HM1.24 antigen as target for multiple myeloma (MM)-directed specific peptide-based immunotherapy. - Methods - The HM1.24 sequence was scanned for immunogenic peptides using the HLA-binding prediction software SYFPEITHI and BIMAS. Peripheral blood mononuclear cells from HLA-A2+ healthy volunteers/blood donors (ND) were stimulated with autologous HM1.24-peptide-loaded dendritic cells, and expanded in vitro. Activation of T cells was assessed by ELISpot and cytotoxicity by 51Chromium (51Cr)-release assays. T2-cells pulsed with irrelevant peptide, the HM1.24−/HLA-A2+ breast carcinoma cell line MCF-7 and the HM1.24+/HLA-A2− myeloma cell line RPMI-8226 were used as controls. Expression of the HM1.24 gene (BST2) was assessed using purified plasma cells and Affymetrix-U133A+B microarrays. Frequency of peptide-specific CD8+ T cells was detected using the flow-cytometric tetramer technique. - Results - Of eight nona-peptides with the highest probability of binding to HLA-A2, the HM1.24 aa22-30 peptide (LLLGIGILV) showed the most frequent activation of CD8+ T cells in healthy volunteers (specific activation in 8 of 11 [73%] ND; compared with 5-19% for the 7 other HM1.24 peptides). Antigen recognition by the HM1.24 aa22-30-specific CD8+ T cells was HLA-A2-restricted (ELISpot with HLA-A2-blocking antibodies: median, 15; range, 14-18 spots/well; isotype-control antibodies: median, 47; range, 44-48). HM1.24-aa22-30-specific CD8+ T cells lysed HLA-A2+ myeloma-derived cell lines (51Cr-release assay: XG-1 vs MCF-7, 91% vs 0%; U266 vs MCF-7, 38% vs 4.2%; IM-9 vs RPMI-8226, 22% vs 0%). Using the cross-reactive Neisseria meningitidis peptide LLSLGIGILV-specific CD8+ T cells recognizing target cells loaded with the HM1.24 aa22-30 peptide (LLLGIGILV) as well as the myeloma-derived cell line U266 could be expanded from MM patients. The HM1.24 gene was expressed at comparable levels by plasma cells from 65 MM patients, 7 patients with monoclonal gammopathy of undetermined significance, and 7 ND. - Conclusions - HM1.24 aa22-30 is a newly identified HLA-A2-restricted T-cell epitope that is processed and presented by major histocompatibility complex class I. Specifically activated CD8+ T cells are able to lyse MM cell lines. We conclude that HM1.24 aa22-30 represents a suitable candidate target for a specific peptide-based immunotherapy of MM.