| |  Online-Ressource |
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| Verfasst von: | Baalmann, Mathis [VerfasserIn]  |
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| | Neises, Laura [VerfasserIn] |
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| | Bitsch, Sebastian [VerfasserIn] |
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| | Schneider, Hendrik [VerfasserIn] |
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| | Deweid, Ludwig Lukas [VerfasserIn] |
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| | Werther, Philipp [VerfasserIn] |
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| | Ilkenhans, Nadja [VerfasserIn] |
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| | Wolfring, Martin [VerfasserIn] |
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| | Ziegler, Michael J. [VerfasserIn] |
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| | Wilhelm, Jonas [VerfasserIn] |
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| | Kolmar, Harald [VerfasserIn] |
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| | Wombacher, Richard [VerfasserIn] |
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| Titel: | A bioorthogonal click chemistry toolbox for targeted synthesis of branched and well-defined protein-protein conjugates |
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| Verf.angabe: | Mathis Baalmann, Laura Neises, Sebastian Bitsch, Hendrik Schneider, Lukas Deweid, Philipp Werther, Nadja Ilkenhans, Martin Wolfring, Michael J. Ziegler, Jonas Wilhelm, Harald Kolmar, and Richard Wombacher |
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| Jahr: | 2020 |
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| Umfang: | 9 S. |
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| Fussnoten: | First published: 28 April 2020 ; Gesehen am 09.11.2021 |
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| Titel Quelle: | Enthalten in: Angewandte Chemie. International edition |
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| Ort Quelle: | Weinheim : Wiley-VCH, 1998 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 59(2020), 31, Seite 12885-12893 |
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| ISSN Quelle: | 1521-3773 |
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| Abstract: | Bioorthogonal chemistry holds great potential to generate difficult-to-access protein-protein conjugate architectures. Current applications are hampered by challenging protein expression systems, slow conjugation chemistry, use of undesirable catalysts, or often do not result in quantitative product formation. Here we present a highly efficient technology for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DAinv). With the aim of precisely generating branched protein chimeras, we systematically assessed the reactivity, stability and side product formation of various bioorthogonal chemistries directly at the protein level. We demonstrate the efficiency and versatility of our conjugation platform using different functional proteins and the therapeutic antibody trastuzumab. This technology enables fast and routine access to tailored and hitherto inaccessible protein chimeras useful for a variety of scientific disciplines. We expect our work to substantially enhance antibody applications such as immunodetection and protein toxin-based targeted cancer therapies. |
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| DOI: | doi:10.1002/anie.201915079 |
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| URL: | kostenfrei: Volltext: https://doi.org/10.1002/anie.201915079 |
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| | kostenfrei: Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201915079 |
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| | DOI: https://doi.org/10.1002/anie.201915079 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | antibody-drug conjugates |
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| | bioorthogonal chemistry |
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| | click chemistry |
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| | protein ligation |
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| | protein-protein conjugates |
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| K10plus-PPN: | 1776541111 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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¬A¬ bioorthogonal click chemistry toolbox for targeted synthesis of branched and well-defined protein-protein conjugates / Baalmann, Mathis [VerfasserIn]; 2020 (Online-Ressource)
