| |  Online-Ressource |
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| Verfasst von: | Selkrig, Joel [VerfasserIn]  |
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| | Stanifer, Megan [VerfasserIn] |
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| | Mateus, André [VerfasserIn] |
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| | Mitosch, Karin [VerfasserIn] |
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| | Barrio-Hernandez, Inigo [VerfasserIn] |
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| | Rettel, Mandy [VerfasserIn] |
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| | Kim, Heeyoung [VerfasserIn] |
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| | Voogdt, Carlos G. P. [VerfasserIn] |
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| | Walch, Philipp [VerfasserIn] |
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| | Kee, Carmon [VerfasserIn] |
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| | Kurzawa, Nils [VerfasserIn] |
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| | Stein, Frank [VerfasserIn] |
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| | Potel, Clément [VerfasserIn] |
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| | Jarzab, Anna [VerfasserIn] |
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| | Kuster, Bernhard [VerfasserIn] |
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| | Bartenschlager, Ralf [VerfasserIn] |
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| | Boulant, Steeve [VerfasserIn] |
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| | Beltrao, Pedro [VerfasserIn] |
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| | Typas, Athanasios [VerfasserIn] |
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| | Savitski, Mikhail M. [VerfasserIn] |
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| Titel: | SARS-CoV-2 infection remodels the host protein thermal stability landscape |
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| Verf.angabe: | Joel Selkrig, Megan Stanifer, André Mateus, Karin Mitosch, Inigo Barrio-Hernandez, Mandy Rettel, Heeyoung Kim, Carlos G.P. Voogdt, Philipp Walch, Carmon Kee, Nils Kurzawa, Frank Stein, Clément Potel, Anna Jarzab, Bernhard Kuster, Ralf Bartenschlager, Steeve Boulant, Pedro Beltrao, Athanasios Typas, Mikhail M. Savitski |
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| E-Jahr: | 2021 |
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| Jahr: | 16 February 2021 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 12.11.2021 |
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| Titel Quelle: | Enthalten in: Molecular systems biology |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2005 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 17(2021), 2, Artikel-ID e10188, Seite 1-13 |
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| ISSN Quelle: | 1744-4292 |
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| Abstract: | Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global threat to human health and has compromised economic stability. In addition to the development of an effective vaccine, it is imperative to understand how SARS-CoV-2 hijacks host cellular machineries on a system-wide scale so that potential host-directed therapies can be developed. In situ proteome-wide abundance and thermal stability measurements using thermal proteome profiling (TPP) can inform on global changes in protein activity. Here we adapted TPP to high biosafety conditions amenable to SARS-CoV-2 handling. We discovered pronounced temporal alterations in host protein thermostability during infection, which converged on cellular processes including cell cycle, microtubule and RNA splicing regulation. Pharmacological inhibition of host proteins displaying altered thermal stability or abundance during infection suppressed SARS-CoV-2 replication. Overall, this work serves as a framework for expanding TPP workflows to globally important human pathogens that require high biosafety containment and provides deeper resolution into the molecular changes induced by SARS-CoV-2 infection. |
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| DOI: | doi:10.15252/msb.202010188 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://dx.doi.org/10.15252/msb.202010188 |
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| | DOI: https://doi.org/10.15252/msb.202010188 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1777372801 |
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| Verknüpfungen: | → Zeitschrift |
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SARS-CoV-2 infection remodels the host protein thermal stability landscape / Selkrig, Joel [VerfasserIn]; 16 February 2021 (Online-Ressource)
