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Verfasst von:Lechner, Christian [VerfasserIn]   i
 Moenning, Ulla [VerfasserIn]   i
 Reichel, Andreas [VerfasserIn]   i
 Fricker, Gert [VerfasserIn]   i
Titel:Potential and limits of kidney cells for evaluation of renal excretion
Verf.angabe:Christian Lechner, Ursula Mönning, Andreas Reichel and Gert Fricker
E-Jahr:2021
Jahr:7 September 2021
Umfang:19 S.
Fussnoten:Gesehen am 15.11.2021
Titel Quelle:Enthalten in: Pharmaceuticals
Ort Quelle:Basel : MDPI, 2004
Jahr Quelle:2021
Band/Heft Quelle:14(2021), 9, Artikel-ID 908, Seite 1-19
ISSN Quelle:1424-8247
Abstract:A large number of therapeutic drugs, herbal components and their metabolites are excreted by the kidneys. Therefore, generally applied models for estimating renal excretion, including freshly isolated rat proximal tubule cells, cultured tubule cells and immortalized kidney cell lines MDCKII, NRK-52E, IHKE-1 and Caki-1, were investigated regarding their predictive potential for active renal transport. Cultured proximal tubule cells showed an epithelial cell-like morphology and formed tight monolayers. However, mRNA expression analyses and immunohistochemical studies revealed patterns of tight junction proteins that were notably different from freshly isolated cells and distinct from those in vivo. High levels of mannitol permeation were found in NRK-52E, IHKE-1 and Caki-1 cells, suggesting that they are not suitable for bidirectional transport studies. Cultured cells and freshly isolated cells also differed in proximal tubule markers and transport proteins, indicating that cultured primary cells were in a state of dedifferentiation. Cell lines MDCKII, NRK-52E, IHKE-1 and Caki-1 did not accurately reflect the characteristics of proximal tubules. The expression patterns of marker and transport proteins differed from freshly isolated primary cells. In summary, each of these models has profound disadvantages to consider when adopting them reliable models for the in vivo situation. Thus, they should not be used alone but only in combination.
DOI:doi:10.3390/ph14090908
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/ph14090908
 Volltext: https://www.mdpi.com/1424-8247/14/9/908
 DOI: https://doi.org/10.3390/ph14090908
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:kidney cell lines
 marker enzymes
 renal excretion
 transport proteins
K10plus-PPN:1777433304
Verknüpfungen:→ Zeitschrift

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