| |  Online-Ressource |
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| Verfasst von: | Hüllein, Jennifer [VerfasserIn]  |
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| | Jethwa, Alexander [VerfasserIn] |
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| | Stolz, Tatjana [VerfasserIn] |
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| | Blume, Carolin [VerfasserIn] |
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| | Sellner, Leopold [VerfasserIn] |
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| | Sill, Martin [VerfasserIn] |
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| | Langer, Christian [VerfasserIn] |
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| | Jauch, Anna [VerfasserIn] |
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| | Paruzynski, Anna [VerfasserIn] |
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| | Kalle, Christof von [VerfasserIn] |
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| | Schmidt, Manfred [VerfasserIn] |
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| | Glimm, Hanno [VerfasserIn] |
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| | Zenz, Thorsten [VerfasserIn] |
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| Titel: | Next-generation sequencing of cancer consensus genes in lymphoma |
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| Verf.angabe: | Jennifer Hüllein, Alexander Jethwa, Tatjana Stolz, Carolin Blume, Leopold Sellner, Martin Sill, Christian Langer, Anna Jauch, Anna Paruzynski, Christof von Kalle, Manfred Schmidt, Hanno Glimm & Thorsten Zenz |
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| E-Jahr: | 2013 |
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| Jahr: | 14 Jun 2013 |
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| Umfang: | 5 S. |
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| Fussnoten: | Gesehen am 16.11.2021 |
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| Titel Quelle: | Enthalten in: Leukemia and lymphoma |
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| Ort Quelle: | London [u.a.] : Taylor & Francis Group, 1989 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 54(2013), 8, Seite 1831-1835 |
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| ISSN Quelle: | 1029-2403 |
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| Abstract: | Sensitive identification of mutations in genes related to the pathogenesis of cancer is a prerequisite for risk-stratified therapies. Next-generation sequencing (NGS) in lymphoma has revealed genetic heterogeneity which makes clinical translation challenging. We established a 454-based targeted resequencing platform for robust high-throughput sequencing from limited material of patients with lymphoma. Hotspot mutations in the most frequently mutated cancer consensus genes were amplified in a two-step multiplex-polymerase chain reation (PCR) which was optimized for homogeneous coverage of all regions of interest. We show that targeted resequencing based on NGS technologies allows highly sensitive detection of mutations and assessment of clone size. The application of this or similar techniques will help the development of genotype-specific treatment approaches in lymphoma. |
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| DOI: | doi:10.3109/10428194.2013.796053 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3109/10428194.2013.796053 |
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| | DOI: https://doi.org/10.3109/10428194.2013.796053 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | cancer consensus genes |
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| | chronic lymphocytic leukemia |
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| | CLL |
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| | lymphoma |
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| | multiple myeloma |
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| | Targeted resequencing |
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| K10plus-PPN: | 1777502640 |
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| Verknüpfungen: | → Zeitschrift |
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Next-generation sequencing of cancer consensus genes in lymphoma / Hüllein, Jennifer [VerfasserIn]; 14 Jun 2013 (Online-Ressource)
