| |  Online-Ressource |
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| Verfasst von: | Winter, Julia [VerfasserIn]  |
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| | Link, Steffen [VerfasserIn] |
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| | Witzigmann, Dominik [VerfasserIn] |
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| | Hildenbrand, Catherina [VerfasserIn] |
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| | Previti, A. Christopher [VerfasserIn] |
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| | Diederichs, Sven [VerfasserIn] |
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| Titel: | Loop-miRs |
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| Titelzusatz: | active microRNAs generated from single-stranded loop regions |
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| Verf.angabe: | Julia Winter, Steffen Link, Dominik Witzigmann, Catherina Hildenbrand, Christopher Previti and Sven Diederichs |
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| E-Jahr: | 2013 |
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| Jahr: | 10 April 2013 |
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| Umfang: | 10 S. |
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| Fussnoten: | Gesehen am 16.11.2021 |
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| Titel Quelle: | Enthalten in: Nucleic acids research |
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| Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 41(2013), 10, Seite 5503-5512 |
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| ISSN Quelle: | 1362-4962 |
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| Abstract: | MicroRNAs (miRNAs) are key mediators of post-transcriptional gene regulation. The miRNA precursors are processed by the endonucleases Drosha and Dicer into a duplex, bound to an Argonaute protein and unwound into two single-stranded miRNAs. Although alternative ways to generate miRNAs have been discovered, e.g. pre-miRNA cleavage by Ago2 or cleavage products of snoRNAs or tRNAs, all known pathways converge on a double-stranded RNA duplex. Exogenous single-stranded siRNAs (ss-siRNAs) can elicit an effective RNA interference reaction; recent studies have identified chemical modifications increasing their stability and activity. Here, we provide first evidence that endogenous, unmodified, single-stranded RNA sequences are generated from single-stranded loop regions of human pre-miRNA hairpins, the so called loop-miRs. Luciferase assays and immunoprecipitation validate loop-miR activity and incorporation into RNA-induced silencing complexes. This study identifies endogenous miRNAs that are generated from single-stranded regions; hence, it provides evidence that precursor-miRNAs can give rise to three distinct endogenous miRNAs: the guide strand, the passenger strand and the loop-miR. |
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| DOI: | doi:10.1093/nar/gkt251 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1093/nar/gkt251 |
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| | DOI: https://doi.org/10.1093/nar/gkt251 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1777549000 |
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| Verknüpfungen: | → Zeitschrift |
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