| Online-Ressource |
Verfasst von: | Winter, Julia [VerfasserIn]  |
| Link, Steffen [VerfasserIn]  |
| Witzigmann, Dominik [VerfasserIn]  |
| Hildenbrand, Catherina [VerfasserIn]  |
| Previti, A. Christopher [VerfasserIn]  |
| Diederichs, Sven [VerfasserIn]  |
Titel: | Loop-miRs |
Titelzusatz: | active microRNAs generated from single-stranded loop regions |
Verf.angabe: | Julia Winter, Steffen Link, Dominik Witzigmann, Catherina Hildenbrand, Christopher Previti and Sven Diederichs |
E-Jahr: | 2013 |
Jahr: | 10 April 2013 |
Umfang: | 10 S. |
Fussnoten: | Gesehen am 16.11.2021 |
Titel Quelle: | Enthalten in: Nucleic acids research |
Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
Jahr Quelle: | 2013 |
Band/Heft Quelle: | 41(2013), 10, Seite 5503-5512 |
ISSN Quelle: | 1362-4962 |
Abstract: | MicroRNAs (miRNAs) are key mediators of post-transcriptional gene regulation. The miRNA precursors are processed by the endonucleases Drosha and Dicer into a duplex, bound to an Argonaute protein and unwound into two single-stranded miRNAs. Although alternative ways to generate miRNAs have been discovered, e.g. pre-miRNA cleavage by Ago2 or cleavage products of snoRNAs or tRNAs, all known pathways converge on a double-stranded RNA duplex. Exogenous single-stranded siRNAs (ss-siRNAs) can elicit an effective RNA interference reaction; recent studies have identified chemical modifications increasing their stability and activity. Here, we provide first evidence that endogenous, unmodified, single-stranded RNA sequences are generated from single-stranded loop regions of human pre-miRNA hairpins, the so called loop-miRs. Luciferase assays and immunoprecipitation validate loop-miR activity and incorporation into RNA-induced silencing complexes. This study identifies endogenous miRNAs that are generated from single-stranded regions; hence, it provides evidence that precursor-miRNAs can give rise to three distinct endogenous miRNAs: the guide strand, the passenger strand and the loop-miR. |
DOI: | doi:10.1093/nar/gkt251 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1093/nar/gkt251 |
| DOI: https://doi.org/10.1093/nar/gkt251 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1777549000 |
Verknüpfungen: | → Zeitschrift |