| |  Online-Ressource |
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| Verfasst von: | Probst, Katja [VerfasserIn]  |
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| | Boutin, Sébastien [VerfasserIn] |
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| | Späth, Isabel [VerfasserIn] |
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| | Scherrer, Martin [VerfasserIn] |
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| | Henny, Nicole [VerfasserIn] |
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| | Sahin, Delal [VerfasserIn] |
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| | Heininger, Alexandra [VerfasserIn] |
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| | Heeg, Klaus [VerfasserIn] |
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| | Nurjadi, Dennis [VerfasserIn] |
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| Titel: | Direct-PCR from rectal swabs and environmental reservoirs |
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| Titelzusatz: | a fast and efficient alternative to detect blaOXA-48 carbapenemase genes in an Enterobacter cloacae outbreak setting |
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| Verf.angabe: | Katja Probst, Sébastien Boutin, Isabel Späth, Martin Scherrer, Nicole Henny, Delal Sahin, Alexandra Heininger, Klaus Heeg, Dennis Nurjadi |
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| Jahr: | 2022 |
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| Umfang: | 5 S. |
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| Fussnoten: | Available online 31 July 2021 ; Im Titel Zusatz zum Titel ist "OXA-48" tiefgestellt ; Gesehen am 18.11.2021 |
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| Titel Quelle: | Enthalten in: Environmental research |
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| Ort Quelle: | San Diego, Calif. : Elsevier, 1967 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 203(2022) vom: Jan., Artikel-ID 111808, Seite 1-5 |
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| ISSN Quelle: | 1096-0953 |
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| Abstract: | Carbapenemase-producing bacteria are a risk factor in clinical settings worldwide. The aim of the study was to accelerate the time to results during an outbreak situation with blaOXA-48-positive Enterobacter cloacae by using a real-time multiplex quantitative PCR (qPCR) directly on rectal swab specimens and on wastewater samples to detect carbapenemase-producing bacteria. Thus, we analyzed 681 rectal swabs and 947 environmental samples during a five-month period by qPCR and compared the results to culture screening. The qPCR showed a sensitivity of 100% by testing directly from rectal swabs and was in ten cases more sensitive than the culture-based methods. Environmental screening for blaOXA-48-carbapenemase genes by qPCR revealed reservoirs of different carbapenemase genes that are potential sources of transmission and might lead to new outbreaks. The rapid identification of patients colonized with those isolates and screening of the hospital environment is essential for earlier patient treatment and eliminating potential sources of nosocomial infections. |
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| DOI: | doi:10.1016/j.envres.2021.111808 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.envres.2021.111808 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0013935121011026 |
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| | DOI: https://doi.org/10.1016/j.envres.2021.111808 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Antimicrobial resistance |
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| | Carbapenemase |
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| | Environmental screening |
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| | Real-time multiplex PCR |
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| K10plus-PPN: | 177798260X |
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| Verknüpfungen: | → Zeitschrift |
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Direct-PCR from rectal swabs and environmental reservoirs / Probst, Katja [VerfasserIn]; 2022 (Online-Ressource)
