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| Verfasst von: | Krieger, Teresa G. [VerfasserIn]  |
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| | Le Blanc, Solange [VerfasserIn] |
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| | Jabs, Julia [VerfasserIn] |
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| | Ten, Foo Wei [VerfasserIn] |
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| | Ishaque, Naveed [VerfasserIn] |
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| | Jechow, Katharina [VerfasserIn] |
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| | Debnath, Olivia [VerfasserIn] |
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| | Leonhardt, Carl-Stephan [VerfasserIn] |
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| | Giri, Anamika [VerfasserIn] |
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| | Eils, Roland [VerfasserIn] |
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| | Strobel, Oliver [VerfasserIn] |
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| | Conrad, Christian [VerfasserIn] |
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| Titel: | Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy |
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| Verf.angabe: | Teresa G. Krieger, Solange Le Blanc, Julia Jabs, Foo Wei Ten, Naveed Ishaque, Katharina Jechow, Olivia Debnath, Carl-Stephan Leonhardt, Anamika Giri, Roland Eils, Oliver Strobel & Christian Conrad |
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| E-Jahr: | 2021 |
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| Jahr: | 05 October 2021 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 23.11.2021 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Springer Nature, 2010 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 12(2021), Artikel-ID 5826, Seite 1-13 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Pancreatic ductal adenocarcinoma (PDAC) is projected to be the second leading cause of cancer mortality by 2030. Bulk transcriptomic analyses have distinguished ‘classical’ from ‘basal-like’ tumors with more aggressive clinical behavior. We derive PDAC organoids from 18 primary tumors and two matched liver metastases, and show that ‘classical’ and ‘basal-like’ cells coexist in individual organoids. By single-cell transcriptome analysis of PDAC organoids and primary PDAC, we identify distinct tumor cell states shared across patients, including a cycling progenitor cell state and a differentiated secretory state. Cell states are connected by a differentiation hierarchy, with ‘classical’ cells concentrated at the endpoint. In an imaging-based drug screen, expression of ‘classical’ subtype genes correlates with better drug response. Our results thus uncover a functional hierarchy of PDAC cell states linked to transcriptional tumor subtypes, and support the use of PDAC organoids as a clinically relevant model for in vitro studies of tumor heterogeneity. |
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| DOI: | doi:10.1038/s41467-021-26059-4 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41467-021-26059-4 |
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| | Volltext: https://www.nature.com/articles/s41467-021-26059-4 |
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| | DOI: https://doi.org/10.1038/s41467-021-26059-4 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cancer genomics |
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| | Experimental models of disease |
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| | Pancreatic cancer |
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| | Tumour heterogeneity |
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| K10plus-PPN: | 1778372279 |
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| Verknüpfungen: | → Zeitschrift |
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Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy / Krieger, Teresa G. [VerfasserIn]; 05 October 2021 (Online-Ressource)
