| |  Online-Ressource |
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| Verfasst von: | Kitiporn Plaimas [VerfasserIn]  |
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| | Wang, Yulin [VerfasserIn] |
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| | Rotimi, Solomon O. [VerfasserIn] |
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| | Olasehinde, Grace [VerfasserIn] |
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| | Fatumo, Segun [VerfasserIn] |
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| | Lanzer, Michael [VerfasserIn] |
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| | Adebiyi, Ezekiel [VerfasserIn] |
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| | König, Rainer [VerfasserIn] |
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| Titel: | Computational and experimental analysis identified 6-diazo-5-oxonorleucine as a potential agent for treating infection by Plasmodium falciparum |
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| Verf.angabe: | Kitiporn Plaimas, Yulin Wang, Solomon O. Rotimi, Grace Olasehinde, Segun Fatumo, Michael Lanzer, Ezekiel Adebiyi, Rainer König |
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| E-Jahr: | 2013 |
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| Jahr: | 9 October 2013 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 25.11.2021 |
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| Titel Quelle: | Enthalten in: Infection, genetics and evolution |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 2001 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 20(2013) vom: Dez., Seite 389-395 |
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| ISSN Quelle: | 1567-7257 |
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| Abstract: | Plasmodium falciparum (PF) is the most severe malaria parasite. It is developing resistance quickly to existing drugs making it indispensable to discover new drugs. Effective drugs have been discovered targeting metabolic enzymes of the parasite. In order to predict new drug targets, computational methods can be used employing database information of metabolism. Using this data, we performed recently a computational network analysis of metabolism of PF. We analyzed the topology of the network to find reactions which are sensitive against perturbations, i.e., when a single enzyme is blocked by drugs. We now used a refined network comprising also the host enzymes which led to a refined set of the five targets glutamyl-tRNA (gln) amidotransferase, hydroxyethylthiazole kinase, deoxyribose-phophate aldolase, pseudouridylate synthase, and deoxyhypusine synthase. It was shown elsewhere that glutamyl-tRNA (gln) amidotransferase of other microorganisms can be inhibited by 6-diazo-5-oxonorleucine. Performing a half maximal inhibitory concentration (IC50) assay, we showed, that 6-diazo-5-oxonorleucine is also severely affecting viability of PF in blood plasma of the human host. We confirmed this by an in vivo study observing Plasmodium berghei infected mice. |
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| DOI: | doi:10.1016/j.meegid.2013.09.019 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.meegid.2013.09.019 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1567134813003602 |
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| | DOI: https://doi.org/10.1016/j.meegid.2013.09.019 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | 6-Diazo-5-oxonorleucine |
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| | Chokepoint |
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| | Malaria |
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| | Metabolic network |
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| K10plus-PPN: | 1779069448 |
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| Verknüpfungen: | → Zeitschrift |
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Computational and experimental analysis identified 6-diazo-5-oxonorleucine as a potential agent for treating infection by Plasmodium falciparum / Kitiporn Plaimas [VerfasserIn]; 9 October 2013 (Online-Ressource)
