| Online-Ressource |
Verfasst von: | Abele, Tobias [VerfasserIn]  |
| Messer, Tobias [VerfasserIn]  |
| Jahnke, Kevin [VerfasserIn]  |
| Hippler, Marc [VerfasserIn]  |
| Bastmeyer, Martin [VerfasserIn]  |
| Wegener, Martin [VerfasserIn]  |
| Göpfrich, Kerstin [VerfasserIn]  |
Titel: | Two-photon 3D laser printing inside synthetic cells |
Verf.angabe: | Tobias Abele, Tobias Messer, Kevin Jahnke, Marc Hippler, Martin Bastmeyer, Martin Wegener & Kerstin Göpfrich |
Jahr: | 2021 |
Umfang: | 15 S. |
Fussnoten: | Gesehen am 01.12.2021 |
Titel Quelle: | Enthalten in: Advanced materials |
Ort Quelle: | Weinheim : Wiley-VCH, 1989 |
Jahr Quelle: | 2021 |
Band/Heft Quelle: | (2021), Artikel-ID 2106709, Seite 1-15 |
ISSN Quelle: | 1521-4095 |
Abstract: | Towards the ambitious goal of manufacturing synthetic cells from the bottom up, various cellular components have already been reconstituted inside of lipid vesicles. However, the deterministic positioning of these components inside the compartment has remained elusive. Here, by using two-photon 3D laser printing, 2D and 3D hydrogel architectures were manufactured with high precision and nearly arbitrary shape inside of preformed giant unilamellar lipid vesicles (GUVs). The required water-soluble photoresist is brought into the GUVs by diffusion in a single mixing step. Crucially, femtosecond two-photon printing inside the compartment does not destroy the GUVs. Beyond this proof-of-principle demonstration, early functional architectures were realized. In particular, a transmembrane structure acting as a pore was 3D printed, thereby allowing for the transport of biological cargo, including DNA, into the synthetic compartment. These experiments show that two-photon 3D laser microprinting can be an important addition to the existing toolbox of synthetic biology. This article is protected by copyright. All rights reserved |
DOI: | doi:10.1002/adma.202106709 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1002/adma.202106709 |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.202106709 |
| DOI: https://doi.org/10.1002/adma.202106709 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Bibliogr. Hinweis: | Forschungsdaten: Abele, Tobias, 1996 - : Two-photon 3D laser printing inside synthetic cells [research data] |
Sach-SW: | 3D laser printing |
| additive manufacturing |
| bottom-up synthetic biology |
| direct laser writing |
| giant unilamellar lipid vesicles (GUVs) |
| PEGDA hydrogel |
| transmembrane pore |
K10plus-PPN: | 1779955189 |
Verknüpfungen: | → Zeitschrift |
Two-photon 3D laser printing inside synthetic cells / Abele, Tobias [VerfasserIn]; 2021 (Online-Ressource)