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Verfasst von:Abele, Tobias [VerfasserIn]   i
 Messer, Tobias [VerfasserIn]   i
 Jahnke, Kevin [VerfasserIn]   i
 Hippler, Marc [VerfasserIn]   i
 Bastmeyer, Martin [VerfasserIn]   i
 Wegener, Martin [VerfasserIn]   i
 Göpfrich, Kerstin [VerfasserIn]   i
Titel:Two-photon 3D laser printing inside synthetic cells
Verf.angabe:Tobias Abele, Tobias Messer, Kevin Jahnke, Marc Hippler, Martin Bastmeyer, Martin Wegener & Kerstin Göpfrich
Jahr:2021
Umfang:15 S.
Fussnoten:Gesehen am 01.12.2021
Titel Quelle:Enthalten in: Advanced materials
Ort Quelle:Weinheim : Wiley-VCH, 1989
Jahr Quelle:2021
Band/Heft Quelle:(2021), Artikel-ID 2106709, Seite 1-15
ISSN Quelle:1521-4095
Abstract:Towards the ambitious goal of manufacturing synthetic cells from the bottom up, various cellular components have already been reconstituted inside of lipid vesicles. However, the deterministic positioning of these components inside the compartment has remained elusive. Here, by using two-photon 3D laser printing, 2D and 3D hydrogel architectures were manufactured with high precision and nearly arbitrary shape inside of preformed giant unilamellar lipid vesicles (GUVs). The required water-soluble photoresist is brought into the GUVs by diffusion in a single mixing step. Crucially, femtosecond two-photon printing inside the compartment does not destroy the GUVs. Beyond this proof-of-principle demonstration, early functional architectures were realized. In particular, a transmembrane structure acting as a pore was 3D printed, thereby allowing for the transport of biological cargo, including DNA, into the synthetic compartment. These experiments show that two-photon 3D laser microprinting can be an important addition to the existing toolbox of synthetic biology. This article is protected by copyright. All rights reserved
DOI:doi:10.1002/adma.202106709
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1002/adma.202106709
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.202106709
 DOI: https://doi.org/10.1002/adma.202106709
Datenträger:Online-Ressource
Sprache:eng
Bibliogr. Hinweis:Forschungsdaten: Abele, Tobias, 1996 - : Two-photon 3D laser printing inside synthetic cells [research data]
Sach-SW:3D laser printing
 additive manufacturing
 bottom-up synthetic biology
 direct laser writing
 giant unilamellar lipid vesicles (GUVs)
 PEGDA hydrogel
 transmembrane pore
K10plus-PPN:1779955189
Verknüpfungen:→ Zeitschrift

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