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| Verfasst von: | Triana, Sergio [VerfasserIn]  |
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| | Vonficht, Dominik [VerfasserIn] |
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| | Jopp-Saile, Lea [VerfasserIn] |
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| | Raffel, Simon [VerfasserIn] |
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| | Lutz, Raphael [VerfasserIn] |
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| | Leonce, Daniel [VerfasserIn] |
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| | Pajonk, Magdalena [VerfasserIn] |
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| | Hernández-Malmierca, Pablo [VerfasserIn] |
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| | Ordoñez-Rueda, Diana [VerfasserIn] |
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| | Ramasz, Beáta [VerfasserIn] |
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| | Boch, Tobias [VerfasserIn] |
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| | Jann, Johann-Christoph [VerfasserIn] |
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| | Nowak, Daniel [VerfasserIn] |
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| | Hofmann, Wolf-Karsten [VerfasserIn] |
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| | Müller-Tidow, Carsten [VerfasserIn] |
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| | Hübschmann, Daniel [VerfasserIn] |
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| | Alexandrov, Theodore [VerfasserIn] |
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| | Benes, Vladimir [VerfasserIn] |
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| | Trumpp, Andreas [VerfasserIn] |
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| | Paulsen, Malte [VerfasserIn] |
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| | Velten, Lars [VerfasserIn] |
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| | Haas, Simon [VerfasserIn] |
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| Titel: | Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states |
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| Verf.angabe: | Sergio Triana, Dominik Vonficht, Lea Jopp-Saile, Simon Raffel, Raphael Lutz, Daniel Leonce, Magdalena Antes, Pablo Hernández-Malmierca, Diana Ordoñez-Rueda, Beáta Ramasz, Tobias Boch, Johann-Christoph Jann, Daniel Nowak, Wolf-Karsten Hofmann, Carsten Müller-Tidow, Daniel Hübschmann, Theodore Alexandrov, Vladimir Benes, Andreas Trumpp, Malte Paulsen, Lars Velten and Simon Haas |
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| E-Jahr: | 2021 |
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| Jahr: | 22 November 2021 |
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| Umfang: | 13 S. |
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| Fussnoten: | Das PDF enthält einen Anhang von 26 Seiten ; Gesehen am 01.12.2021 |
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| Titel Quelle: | Enthalten in: Nature immunology |
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| Ort Quelle: | London : Springer Nature Limited, 2000 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 22(2021), 12, Seite 1577-1589 |
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| ISSN Quelle: | 1529-2916 |
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| Abstract: | Single-cell genomics technology has transformed our understanding of complex cellular systems. However, excessive cost and a lack of strategies for the purification of newly identified cell types impede their functional characterization and large-scale profiling. Here, we have generated high-content single-cell proteo-genomic reference maps of human blood and bone marrow that quantitatively link the expression of up to 197 surface markers to cellular identities and biological processes across all main hematopoietic cell types in healthy aging and leukemia. These reference maps enable the automatic design of cost-effective high-throughput cytometry schemes that outperform state-of-the-art approaches, accurately reflect complex topologies of cellular systems and permit the purification of precisely defined cell states. The systematic integration of cytometry and proteo-genomic data enables the functional capacities of precisely mapped cell states to be measured at the single-cell level. Our study serves as an accessible resource and paves the way for a data-driven era in cytometry. |
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| DOI: | doi:10.1038/s41590-021-01059-0 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41590-021-01059-0 |
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| | Volltext: https://www.nature.com/articles/s41590-021-01059-0 |
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| | DOI: https://doi.org/10.1038/s41590-021-01059-0 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Gene expression analysis |
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| | Haematopoiesis |
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| | Haematopoietic stem cells |
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| | Leukaemia |
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| K10plus-PPN: | 1779975961 |
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| Verknüpfungen: | → Zeitschrift |
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Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states / Triana, Sergio [VerfasserIn]; 22 November 2021 (Online-Ressource)
