Synthesis of nanostructured and biofunctionalized water-in-oil droplets as tools for homing T cells

• Verfasst von: Platzman, Ilia [VerfasserIn]
• Verfasst von: Janiesch, Jan-Willi [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Synthesis of nanostructured and biofunctionalized water-in-oil droplets as tools for homing T cells
• Verf.angabe: Ilia Platzman, Jan-Willi Janiesch, Joachim Pius Spatz
• E-Jahr: 2013
• Jahr: February 18, 2013
• Umfang: 4 S.
• Fussnoten: Gesehen am 02.12.2021
• Titel Quelle: Enthalten in: American Chemical SocietyJournal of the American Chemical Society
• Ort Quelle: Washington, DC : ACS Publications, 1879
• Jahr Quelle: 2013
• Band/Heft Quelle: 135(2013), 9, Seite 3339-3342
• ISSN Quelle: 1520-5126
• DOI: doi:10.1021/ja311588c
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 178007638X

Abstract

Activation, ex vivo expansion of T cells, differentiation into a regulatory subset, and its phenotype-specific high-throughput selection represent major challenges in immunobiology. In part, this is due to the lack of technical means to synthesize suitable 3D extracellular systems to imitate ex vivo the cellular interactions between T cells and antigen-presenting cells (APCs). In this study, we synthesized a new type of gold-linked surfactant and used a drop-based microfluidic device to develop and characterize novel nanostructured and specifically biofunctionalized droplets of water-in-oil emulsions as 3D APC analogues. Combining flexible biofunctionalization with the pliable physical properties of the nanostructured droplets provided this system with superior properties in comparison with previously reported synthetic APC analogues.