| |  Online-Ressource |
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| Verfasst von: | Pape, Constantin [VerfasserIn]  |
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| | Remme, Roman [VerfasserIn] |
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| | Wolny, Adrian [VerfasserIn] |
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| | Olberg, Sylvia [VerfasserIn] |
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| | Wolf, Steffen [VerfasserIn] |
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| | Cerrone, Lorenzo [VerfasserIn] |
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| | Cortese, Mirko [VerfasserIn] |
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| | Klaus, Severina [VerfasserIn] |
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| | Lucic, Bojana [VerfasserIn] |
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| | Ullrich, Stephanie [VerfasserIn] |
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| | Anders-Ößwein, Maria [VerfasserIn] |
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| | Wolf, Stefanie [VerfasserIn] |
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| | Cerikan, Berati [VerfasserIn] |
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| | Neufeldt, Christopher [VerfasserIn] |
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| | Ganter, Markus [VerfasserIn] |
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| | Schnitzler, Paul [VerfasserIn] |
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| | Merle, Uta [VerfasserIn] |
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| | Lusic, Marina [VerfasserIn] |
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| | Boulant, Steeve [VerfasserIn] |
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| | Stanifer, Megan [VerfasserIn] |
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| | Bartenschlager, Ralf [VerfasserIn] |
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| | Hamprecht, Fred [VerfasserIn] |
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| | Kreshuk, Anna [VerfasserIn] |
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| | Tischer, Christian [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| | Müller, Barbara [VerfasserIn] |
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| | Laketa, Vibor [VerfasserIn] |
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| Titel: | Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera |
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| Verf.angabe: | Constantin Pape, Roman Remme, Adrian Wolny, Sylvia Olberg, Steffen Wolf, Lorenzo Cerrone, Mirko Cortese, Severina Klaus, Bojana Lucic, Stephanie Ullrich, Maria Anders-Össwein, Stefanie Wolf, Berati Cerikan, Christopher J. Neufeldt, Markus Ganter, Paul Schnitzler, Uta Merle, Marina Lusic, Steeve Boulant, Megan Stanifer, Ralf Bartenschlager, Fred A. Hamprecht, Anna Kreshuk, Christian Tischer, Hans-Georg Kräusslich, Barbara Müller, Vibor Laketa |
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| Jahr: | 2021 |
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| Umfang: | 15 S. |
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| Fussnoten: | First published: 30 December 2020 ; Gesehen am 02.12.2021 |
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| Titel Quelle: | Enthalten in: Bioessays |
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| Ort Quelle: | New York, NY : Wiley-Liss, 1984 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 43(2021), 3, Artikel-ID 2000257, Seite 1-15 |
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| ISSN Quelle: | 1521-1878 |
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| Abstract: | Emergence of the novel pathogenic coronavirus SARS-CoV-2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi-quantitative high-content microscopy-based assay for detection of three major classes (IgG, IgA, and IgM) of SARS-CoV-2 specific antibodies in human samples. The possibility to detect antibodies against the entire viral proteome together with a robust semi-automated image analysis workflow resulted in specific, sensitive and unbiased assay that complements the portfolio of SARS-CoV-2 serological assays. Sensitive, specific and quantitative serological assays are urgently needed for a better understanding of humoral immune response against the virus as a basis for developing public health strategies to control viral spread. The procedure described here has been used for clinical studies and provides a general framework for the application of quantitative high-throughput microscopy to rapidly develop serological assays for emerging virus infections. |
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| DOI: | doi:10.1002/bies.202000257 |
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| URL: | kostenfrei: Volltext ; Verlag: https://doi.org/10.1002/bies.202000257 |
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| | kostenfrei: Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/bies.202000257 |
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| | DOI: https://doi.org/10.1002/bies.202000257 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | antibody |
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| | immunofluorescence |
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| | machine learning image analysis |
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| | quantitative microscopy |
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| | SARS-CoV-2 |
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| | serological test |
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| K10plus-PPN: | 1780082096 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera / Pape, Constantin [VerfasserIn]; 2021 (Online-Ressource)
