| |  Online-Ressource |
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| Verfasst von: | Herbst, Sophie [VerfasserIn]  |
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| | Stolarczyk, Marta [VerfasserIn] |
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| | Becirovic, Tina [VerfasserIn] |
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| | Czernilofsky, Felix [VerfasserIn] |
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| | Liu, Yi [VerfasserIn] |
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| | Kolb, Carolin [VerfasserIn] |
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| | Knoll, Mareike [VerfasserIn] |
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| | Herling, Marco [VerfasserIn] |
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| | Müller-Tidow, Carsten [VerfasserIn] |
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| | Dietrich, Sascha [VerfasserIn] |
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| Titel: | Phagocytosis by stroma confounds coculture studies |
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| Verf.angabe: | Sophie A. Herbst, Marta Stolarczyk, Tina Becirovic, Felix Czernilofsky, Yi Liu, Carolin Kolb, Mareike Knoll, Marco Herling, Carsten Müller-Tidow, and Sascha Dietrich |
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| E-Jahr: | 2021 |
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| Jahr: | 30 August 2021 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 02.12.2021 |
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| Titel Quelle: | Enthalten in: iScience |
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| Ort Quelle: | Amsterdam : Elsevier, 2018 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 24(2021), 9, Artikel-ID 103062, Seite 1-15 |
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| ISSN Quelle: | 2589-0042 |
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| Abstract: | Signals provided by the microenvironment can modify and circumvent pathway activities that are therapeutically targeted by drugs. Bone marrow stromal cell coculture models are frequently used to study the influence of the bone marrow niche on ex vivo drug response. Here, we show that mesenchymal stromal cells from selected donors and NKTert, a stromal cell line, which is commonly used for coculture studies with primary leukemia cells, extensively phagocytose apoptotic cells. This could lead to misinterpretation of results, especially if viability readouts of the target cells (e.g. leukemic cells) in such coculture models are based on the relative proportions of dead and alive cells. Future coculture studies which aim to investigate the impact of bone marrow stromal cells on drug response should take into account that stromal cells have the capacity to phagocytose apoptotic cells. |
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| DOI: | doi:10.1016/j.isci.2021.103062 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.isci.2021.103062 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S2589004221010300 |
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| | DOI: https://doi.org/10.1016/j.isci.2021.103062 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Biological Sciences |
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| | Cancer |
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| | Cell Biology |
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| | Stem Cells Research |
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| K10plus-PPN: | 1780151772 |
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| Verknüpfungen: | → Zeitschrift |
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Phagocytosis by stroma confounds coculture studies / Herbst, Sophie [VerfasserIn]; 30 August 2021 (Online-Ressource)
