Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach

• Verfasst von: Pohl, Theresa Lucie Maria [VerfasserIn]
• Verfasst von: Baha-Schwab, Elisabeth H. [VerfasserIn]
• Verfasst von: Cavalcanti-Adam, Elisabetta A. [VerfasserIn]
• Titel: Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach
• Verf.angabe: Theresa L.M. Pohl, Elisabeth H. Schwab, Elisabetta A. Cavalcanti-Adam
• Jahr: 2013
• Umfang: 1 Online-Ressource (1 Videodatei, 2:00 min)
• Illustrationen: farbig
• Fussnoten: Enthält auch Textversion ; Gesehen am 03.12.2021 ; Wissenschaftlicher Film. Deutschland. 2013
• Titel Quelle: Enthalten in: JoVE. Video journal
• Ort Quelle: [S.l.] : [s.n.], 2006
• Jahr Quelle: 2013
• Band/Heft Quelle: (2013), 78, Artikel-ID e50842, Seite ?
• ISSN Quelle: 1940-087X
• DOI: doi:10.3791/50842
• Datenträger: Online-Ressource
• Dokumenttyp: Film
• Sprache: eng
• K10plus-PPN: 1780187114

Abstract

Bone morphogenetic protein 2 (BMP-2) is a growth factor embedded in the extracellular matrix of bone tissue. BMP-2 acts as trigger of mesenchymal cell differentiation into osteoblasts, thus stimulating healing and de novo bone formation. The clinical use of recombinant human BMP-2 (rhBMP-2) in conjunction with scaffolds has raised recent controversies, based on the mode of presentation and the amount to be delivered. The protocol presented here provides a simple and efficient way to deliver BMP-2 for in vitro studies on cells. We describe how to form a self-assembled monolayer consisting of a heterobifunctional linker, and show the subsequent binding step to obtain covalent immobilization of rhBMP-2. With this approach it is possible to achieve a sustained presentation of BMP-2 while maintaining the biological activity of the protein. In fact, the surface immobilization of BMP-2 allows targeted investigations by preventing unspecific adsorption, while reducing the amount of growth factor and, most notably, hindering uncontrolled release from the surface. Both short- and long-term signaling events triggered by BMP-2 are taking place when cells are exposed to surfaces presenting covalently immobilized rhBMP-2, making this approach suitable for in vitro studies on cell responses to BMP-2 stimulation.