Tuning the volume of the immune response

• Verfasst von: Luft, Thomas [VerfasserIn]
• Verfasst von: Maraskovsky, Eugene [VerfasserIn]
• Verfasst von: Schnurr, Max [VerfasserIn]
• Verfasst von: Knebel, Katja [VerfasserIn]
• Verfasst von: Kirsch, Michael [VerfasserIn]
• Verfasst von: Görner, Martin [VerfasserIn]
• Verfasst von: Skoda, Radek [VerfasserIn]
• Verfasst von: Ho, Anthony Dick [VerfasserIn]
• Verfasst von: Nawroth, Peter Paul [VerfasserIn]
• Verfasst von: Bierhaus, Angelika [VerfasserIn]
• Titel: Tuning the volume of the immune response
• Titelzusatz: strength and persistence of stimulation determine migration and cytokine secretion of dendritic cells
• Verf.angabe: Thomas Luft, Eugene Maraskovsky, Max Schnurr, Katja Knebel, Michael Kirsch, Martin Görner, Radek Skoda, Anthony D. Ho, Peter Nawroth, and Angelika Bierhaus
• E-Jahr: 2004
• Jahr: [15 August 2004]
• Umfang: 9 S.
• Illustrationen: Illustrationen
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe ; Gesehen am 03.12.2021
• Titel Quelle: Enthalten in: Blood
• Ort Quelle: Washington, DC : American Society of Hematology, 1946
• Jahr Quelle: 2004
• Band/Heft Quelle: 104(2004), 4, Seite 1066-1074
• ISSN Quelle: 1528-0020
• DOI: doi:10.1182/blood-2003-12-4146
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1780202059

Abstract

Migration to lymph nodes and secretion of cytokines are critical functions of mature dendritic cells (DCs); however, these 2 functions are not necessarily linked. This is the first report showing that quantitative differences in identical signaling pathways determine DC migration and cytokine secretion. Using different polymerized forms of CD40 ligand, we demonstrate that the strength and persistence of CD40 signaling can induce either function. Induction of monocyte-derived DC (MoDC) migration required a weak and transient CD40 signal, whereas strong and persistent CD40 signaling blocked migration and biased toward cytokine secretion. In contrast to MoDCs, CD40 activation of CD1c+ peripheral blood DCs (PBDCs) induced a nonpersistent, intracellular signaling profile resulting in migratory-type DCs unable to secrete interleukin-12p70 (IL-12p70). Extracellular signal-regulated kinase 1/2 (ERK1/2) and p38K activation synergistically mediated cytokine secretion, whereas migration was enhanced by p38K activation but reduced by persistent ERK1/2 activity. This model of signal strength and persistence also applied when stimulating DCs with intact microbes. Thus, a novel concept emerges in which the type of immune response induced by DCs is tuned by the strength and persistence of DC activating signals.