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Verfasst von:Preusser, Matthias [VerfasserIn]   i
 Capper, David [VerfasserIn]   i
 Berghoff, Anna S. [VerfasserIn]   i
 Horvat, Reinhard [VerfasserIn]   i
 Wrba, Fritz [VerfasserIn]   i
 Schindl, Monika [VerfasserIn]   i
 Schoppmann, Sebastian F. [VerfasserIn]   i
 Deimling, Andreas von [VerfasserIn]   i
 Birner, Peter [VerfasserIn]   i
Titel:Expression of BRAF V600E mutant protein in epithelial ovarian tumors
Verf.angabe:Matthias Preusser MD; David Capper MD; Anna S. Berghoff MD; Reinhard Horvat MD; Fritz Wrba MD; Monika SchindlMD; Sebastian F. Schoppmann MD; Andreas von Deimling MD; Peter Birner MD, MSc
Jahr:2013
Umfang:6 S.
Fussnoten:Gesehen am 09.12.2021
Titel Quelle:Enthalten in: Applied immunohistochemistry & molecular morphology
Ort Quelle:Philadelphia, Pa. : Lippincott Williams & Wilkins, 1999
Jahr Quelle:2013
Band/Heft Quelle:21(2013), 2, Seite 159-164
ISSN Quelle:1533-4058
Abstract:Background: Genetic analyses have identified BRAF V600E mutations in a subset of ovarian carcinomas. The aim of this study was to investigate the expression of BRAF V600E aberrant protein using a novel mutation-specific antibody in epithelial ovarian tumors. Methods: We immunohistochemically analyzed expression of V600E-mutant BRAF protein in archival formalin-fixed, paraffin-embedded tissue specimens of 142 epithelial ovarian tumors [98 invasive carcinomas and 44 tumors of low malignant potential (LMP)] using monoclonal antibody VE1. BRAF mutation status was validated in all immunopositive cases and in 6 immunonegative control cases by gene sequencing. Results: We found anti-BRAF V600E immunolabeling in 4 serous carcinomas and 5 serous LMP. Presence of a BRAF V600E mutation was confirmed by sequencing analysis in 6 of the 9 cases (3 LMP tumors, 3 low-grade carcinomas). In 2 partially VE1-positive tumors deriving from 1 patient (1 LMP and 1 contralateral invasive high-grade serous carcinoma), genetic analysis repeatedly resulted in BRAF wild-type, arguing for false-positive immunostaining results. One immunopositive case was repeatedly inconclusive in genetic analysis. In all 6 genetically confirmed cases, BRAF V600E mutant protein expression was homogenous throughout the tumor tissue. Conclusions: We found BRAF V600E mutations in 13% (4/31) of serous LMP and 5% (3/62) of invasive serous carcinomas. No BRAF V600E mutations were detected in nonserous epithelial ovarian tumors. For reliable assessment of the BRAF V600E status in ovarian epithelial tumor samples, an integrated approach using immunohistochemistry and genetic analysis seems advisable, as both methods lead to incorrect results in some cases.
DOI:doi:10.1097/PAI.0b013e31825d7402
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1097/PAI.0b013e31825d7402
 Volltext: https://journals.lww.com/appliedimmunohist/Abstract/2013/03000/Expression_of_BRAF_V600E_Mutant_Protein_in.9.aspx
 DOI: https://doi.org/10.1097/PAI.0b013e31825d7402
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1780817584
Verknüpfungen:→ Zeitschrift

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