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| Verfasst von: | Loconte, Valentina [VerfasserIn]  |
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| | Chen, Jian-Hua [VerfasserIn] |
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| | Cortese, Mirko [VerfasserIn] |
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| | Ekman, Axel [VerfasserIn] |
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| | Le Gros, Mark A. [VerfasserIn] |
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| | Larabell, Carolyn [VerfasserIn] |
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| | Bartenschlager, Ralf [VerfasserIn] |
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| | Weinhardt, Venera [VerfasserIn] |
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| Titel: | Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells |
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| Verf.angabe: | Valentina Loconte, Jian-Hua Chen, Mirko Cortese, Axel Ekman, Mark A. Le Gros, Carolyn Larabell, Ralf Bartenschlager, and Venera Weinhardt |
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| E-Jahr: | 2021 |
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| Jahr: | October 28, 2021 |
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| Umfang: | 16 S. |
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| Fussnoten: | Gesehen am 09.12.2021 |
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| Titel Quelle: | Enthalten in: Cell reports. Methods |
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| Ort Quelle: | Cambridge, MA : Cell Press, 2021 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 1(2021), 7 vom: Nov., Artikel-ID 100117, Seite 1-13, e1-3 |
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| ISSN Quelle: | 2667-2375 |
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| Abstract: | High-resolution and rapid imaging of host cell ultrastructure can generate insights toward viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Here, we employ full-rotation soft X-ray tomography (SXT) to examine organelle remodeling induced by SARS-CoV-2 at the whole-cell level with high spatial resolution and throughput. Most of the current SXT systems suffer from a restricted field of view due to use of flat sample supports and artifacts due to missing data. In this approach using cylindrical sample holders, a full-rotation tomogram of human lung epithelial cells is performed in less than 10 min. We demonstrate the potential of SXT imaging by visualizing aggregates of SARS-CoV-2 virions and virus-induced intracellular alterations. This rapid whole-cell imaging approach allows us to visualize the spatiotemporal changes of cellular organelles upon viral infection in a quantitative manner. |
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| DOI: | doi:10.1016/j.crmeth.2021.100117 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.crmeth.2021.100117 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S2667237521001818 |
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| | DOI: https://doi.org/10.1016/j.crmeth.2021.100117 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | cells |
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| | cryo-tomography |
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| | organelles |
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| | SARS-CoV-2 |
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| | soft X-ray |
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| | synchrotron |
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| | tomography |
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| | viral replication organelles |
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| K10plus-PPN: | 1780818661 |
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| Verknüpfungen: | → Zeitschrift |
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Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells / Loconte, Valentina [VerfasserIn]; October 28, 2021 (Online-Ressource)
