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Status: Bibliographieeintrag

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Verfasst von:Jakob, Dorothee [VerfasserIn]   i
 Klesen, Alexander [VerfasserIn]   i
 Allegrini, Benoit [VerfasserIn]   i
 Darkow, Elisa [VerfasserIn]   i
 Aria, Diana [VerfasserIn]   i
 Emig, Ramona [VerfasserIn]   i
 Chica, Ana Simon [VerfasserIn]   i
 Rog-Zielinska, Eva A. [VerfasserIn]   i
 Guth, Tim [VerfasserIn]   i
 Beyersdorf, Friedhelm [VerfasserIn]   i
 Kari, Fabian A. [VerfasserIn]   i
 Proksch, Susanne [VerfasserIn]   i
 Hatem, Stéphane N. [VerfasserIn]   i
 Karck, Matthias [VerfasserIn]   i
 Künzel, Stephan R. [VerfasserIn]   i
 Guizouarn, Hélène [VerfasserIn]   i
 Schmidt, Constanze [VerfasserIn]   i
 Kohl, Peter [VerfasserIn]   i
 Ravens, Ursula [VerfasserIn]   i
 Peyronnet, Rémi [VerfasserIn]   i
Titel:Piezol and BKca channels in human atrial fibroblasts
Titelzusatz:Interplay and remodelling in atrial fibrillation
Verf.angabe:Dorothee Jakob, Alexander Klesen, Benoit Allegrini, Elisa Darkow, Diana Aria, Ramona Emig, Ana Simon Chica, Eva A. Rog-Zielinska, Tim Guth, Friedhelm Beyersdorf, Fabian A. Kari, Susanne Proksch, Stéphane N. Hatem, Matthias Karck, Stephan R. Künzel, Hélène Guizouarn, Constanze Schmidt, Peter Kohl, Ursula Ravens, Rémi Peyronnet
E-Jahr:2021
Jahr:8 May 2021
Umfang:14 S.
Fussnoten:Im Titel sind die Zeichen "ca" tiefgestellt ; Gesehen am 09.12.2021
Titel Quelle:Enthalten in: Journal of molecular and cellular cardiology
Ort Quelle:New York, NY [u.a.] : Elsevier, 1970
Jahr Quelle:2021
Band/Heft Quelle:158(2021), Seite 49-62
ISSN Quelle:1095-8584
Abstract:Aims: Atrial Fibrillation (AF) is an arrhythmia of increasing prevalence in the aging populations of developed countries. One of the important indicators of AF is sustained atrial dilatation, highlighting the importance of mechanical overload in the pathophysiology of AF. The mechanisms by which atrial cells, including fibroblasts, sense and react to changing mechanical forces, are not fully elucidated. Here, we characterise stretch-activated ion channels (SAC) in human atrial fibroblasts and changes in SAC- presence and activity associated with AF. Methods and results: Using primary cultures of human atrial fibroblasts, isolated from patients in sinus rhythm or sustained AF, we combine electrophysiological, molecular and pharmacological tools to identify SAC. Two electrophysiological SAC- signatures were detected, indicative of cation-nonselective and potassium-selective channels. Using siRNA-mediated knockdown, we identified the cation-nonselective SAC as Piezol. Biophysical properties of the potassium-selective channel, its sensitivity to calcium, paxilline or iberiotoxin (blockers), and N511021 (activator), indicated presence of calcium-dependent 'big potassium channels' (BKca). In cells from AF patients, Piezol activity and mRNA expression levels were higher than in cells from sinus rhythm patients, while BKca activity (but not expression) was downregulated. Both Piezol-knockdown and removal of extracellular calcium from the patch pipette resulted in a significant reduction of BKca current during stretch. No co-immunoprecipitation of Piezol and BKca was detected. Conclusions: Human atrial fibroblasts contain at least two types of ion channels that are activated during stretch: Piezol and BKca. While Piezol is directly stretch-activated, the increase in BKca activity during mechanical stimulation appears to be mainly secondary to calcium influx via SAC such as Piezol. During sustained AF, Piezol is increased, while BKca activity is reduced, highlighting differential regulation of both channels. Our data support the presence and interplay of Piezol and BKca in human atrial fibroblasts in the absence of physical links between the two channel proteins.
DOI:doi:10.1016/j.yjmcc.2021.05.002
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.yjmcc.2021.05.002
 Volltext: https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=DOISource&SrcApp=WOS&KeyAID=10.1016%2Fj.yjmcc ...
 DOI: https://doi.org/10.1016/j.yjmcc.2021.05.002
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Arrhythmia
 Calcium
 dilatation
 duration
 heart
 Heart
 infarction
 interleukin-6
 ion channels
 k+ channel
 kcnma1
 Mechano-sensing
 mechanoelectric feedback
 membrane stretch
 Non-myocytes
 Stretch-activated ion channels
 vulnerability
K10plus-PPN:1780835086
Verknüpfungen:→ Zeitschrift

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