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Verfasst von:Huang, Shiang [VerfasserIn]   i
 Law, Ping [VerfasserIn]   i
 Francis, Karl [VerfasserIn]   i
 Palsson, Bernhard O. [VerfasserIn]   i
 Ho, Anthony Dick [VerfasserIn]   i
Titel:Symmetry of initial cell divisions among primitive hematopoietic progenitors is independent of ontogenic age and regulatory molecules
Verf.angabe:Shiang Huang, Ping Law, Karl Francis, Bernhard O. Palsson, and Anthony D. Ho
E-Jahr:1999
Jahr:[15 October 1999]
Umfang:10 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 10.12.2021
Titel Quelle:Enthalten in: Blood
Ort Quelle:Washington, DC : American Society of Hematology, 1946
Jahr Quelle:1999
Band/Heft Quelle:94(1999), 8, Seite 2595-2604
ISSN Quelle:1528-0020
Abstract:We have developed a time-lapse camera system to follow the replication history and the fate of hematopoietic stem cells (HSC) at a single-cell level. Combined with single-cell culture, we correlated the early replication behavior with colony development after 14 days. The membrane dye PKH26 was used to monitor cell division. In addition to multiple, synchronous, and symmetric divisions, single-sorted CD34+/CD38− cells derived from fetal liver (FLV) also gave rise to a daughter cell that remained quiescent for up to 8 days, whereas the other daughter cell proliferated exponentially. Upon separation and replating as single cells onto medium containing a cytokine cocktail, 60.6% ± 9.8% of the initially quiescent cells (PKH26 bright) gave rise again to colonies and 15.8% ± 7.8% to blast colonies that could be replated. We have then determined the effects of various regulatory molecules on symmetry of initial cell divisions. After single-cell sorting, the CD34+/CD38− cells derived from FLV were exposed to flt3-ligand, thrombopoietin, stem cell factor (SCF), or medium containing a cytokine cocktail (with SCF, interleukin-3, interleukin-6, granulocyte-macrophage colony-stimulating factor, and erythropoietin). Whereas mitotic rate, colony efficiency, and asymmetric divisions could be altered using various regulatory molecules, the asymmetric division index, defined as the number of asymmetric divisions versus the number of dividing cells, was not altered significantly. This observation suggests that, although lineage commitment and cell proliferation can be skewed by extrinsic signaling, symmetry of early divisions is probably under the control of intrinsic factors.
DOI:doi:10.1182/blood.V94.8.2595.420k37_2595_2604
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1182/blood.V94.8.2595.420k37_2595_2604
 Volltext: https://www.sciencedirect.com/science/article/pii/S0006497120711109
 DOI: https://doi.org/10.1182/blood.V94.8.2595.420k37_2595_2604
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1780993439
Verknüpfungen:→ Zeitschrift

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