Role of Rck-Pat1b binding in assembly of processing-bodies

• Verfasst von: Özgür, Sevim [VerfasserIn]
• Verfasst von: Stoecklin, Georg [VerfasserIn]
• Titel: Role of Rck-Pat1b binding in assembly of processing-bodies
• Verf.angabe: Sevim Ozgur & Georg Stoecklin
• E-Jahr: 2013
• Jahr: 27 Mar 2013
• Umfang: 12 S.
• Fussnoten: Gesehen am 13.12.2021
• Titel Quelle: Enthalten in: RNA biology
• Ort Quelle: Philadelphia, Pa. : Taylor & Francis, 2004
• Jahr Quelle: 2013
• Band/Heft Quelle: 10(2013), 4, Seite 528-539
• ISSN Quelle: 1555-8584
• DOI: doi:10.4161/rna.24086
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: decapping
• Sach-SW: mRNA degradation
• Sach-SW: PatL1/Pat1b
• Sach-SW: processing body
• Sach-SW: Rck/p54
• Sach-SW: RNA helicase
• Sach-SW: RNA-induced silencing complex
• Sach-SW: translation suppression
• K10plus-PPN: 1781999732

Abstract

The DEAD box RNA helicase Rck and the scaffold protein Pat1b participate in controlling gene expression at the post-transcriptional level by suppressing mRNA translation and promoting mRNA decapping. In addition, both proteins are required for the assembly of processing (P)-bodies, cytoplasmic foci that contain stalled mRNAs and numerous components of the mRNA decay machinery. The C-terminal RecA-like domain of Rck interacts with the N-terminal acidic domain of Pat1b. Here, we identified point mutations in human Rck and Pat1b that prevent the two proteins from binding to each other. By analyzing interaction-deficient mutants in combination with knockdown and rescue strategies in human HeLa cells, we found that Pat1b assembles P-bodies and suppresses expression of tethered mRNAs in the absence of Rck binding. In contrast, Rck requires the Pat1b-binding site in order to promote P-body assembly and associate with the decapping enzyme Dcp2 as well as Ago2 and TNRC6A, two core components of the RNA-induced silencing complex. Our data indicate that P-body assembly occurs in a step-wise manner, where Rck participates in the initial suppression of mRNA translation, whereas Pat1b in a second step triggers P-body assembly and promotes mRNA decapping.