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Status: Bibliographieeintrag

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Verfasst von:Radovits, Tamás [VerfasserIn]   i
 Arif, Rawa [VerfasserIn]   i
 Bömicke, Timo [VerfasserIn]   i
 Korkmaz-İçöz, Sevil [VerfasserIn]   i
 Barnucz, Enikő [VerfasserIn]   i
 Karck, Matthias [VerfasserIn]   i
 Merkely, Béla [VerfasserIn]   i
 Szabó, Gábor [VerfasserIn]   i
Titel:Vascular dysfunction induced by hypochlorite is improved by the selective phosphodiesterase-5-inhibitor vardenafil
Verf.angabe:Tamás Radovits, Rawa Arif, Timo Bömicke, Sevil Korkmaz, Enikő Barnucz, Matthias Karck, Béla Merkely, Gábor Szabó
E-Jahr:2013
Jahr:23 April 2013
Umfang:10 S.
Fussnoten:Gesehen am 13.12.2021
Titel Quelle:Enthalten in: European journal of pharmacology
Ort Quelle:New York, NY [u.a.] : Elsevier, 1967
Jahr Quelle:2013
Band/Heft Quelle:710(2013), 1/3 vom: 15. Juni, Seite 110-119
ISSN Quelle:1879-0712
Abstract:Reactive oxygen species, such as hypochlorite induce oxidative stress, which impairs nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) signalling and leads to vascular dysfunction. It has been proposed, that elevated cGMP-levels may contribute to an effective cytoprotection against oxidative stress. We investigated the effects of vardenafil, a selective inhibitor of the cGMP-degrading phosphodiesterase-5 enzyme on vascular dysfunction induced by hypochlorite. In organ bath experiments for isometric tension, we investigated the endothelium-dependent and endothelium-independent vasorelaxation of isolated rat aortic rings using cumulative concentrations of acetylcholine and sodium nitroprusside (SNP). Vascular dysfunction was induced by exposing rings to hypochlorite (100-400 µM). In the treatment groups, rats were pretreated with vardenafil (30 and 300 µg/kg i.v.). Immunohistochemical analysis was performed for the oxidative stress markers nitrotyrosine, poly(ADP-ribose) and for apoptosis inducing factor (AIF). Exposure to hypochlorite resulted in a marked impairment of acetylcholine-induced endothelium-dependent vasorelaxation of aortic rings. Pretreatment with vardenafil led to improved endothelial function as reflected by the higher maximal vasorelaxation (Rmax) to acetylcholine. Regarding endothelium-independent vasorelaxation, hypochlorite exposure led to a left-shift of SNP concentration-response curves in the vardenafil groups without any alterations of the Rmax. In the hypochlorite groups immunohistochemical analysis showed enhanced poly(ADP-ribose)-formation and nuclear translocation of AIF, which were prevented by vardenafil-pretreatment. Our results support the view that cytoprotective effects of PDE-5-inhibitors on the endothelium may underlie the improved endothelial function, however, a slight sensitisation of vascular smooth muscle to NO was also confirmed. PDE-5-inhibition may represent a potential therapy approach for treating vascular dysfunction induced by oxidative stress.
DOI:doi:10.1016/j.ejphar.2013.04.012
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.ejphar.2013.04.012
 Volltext: https://www.sciencedirect.com/science/article/pii/S0014299913003051?via%3Dihub
 DOI: https://doi.org/10.1016/j.ejphar.2013.04.012
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Acetylcholine
 Animals
 Aorta, Thoracic
 Endothelium, Vascular
 Hypochlorous Acid
 Imidazoles
 In Vitro Techniques
 Male
 Nitroprusside
 Oxidants
 Oxidative Stress
 Phenylephrine
 Phosphodiesterase 5 Inhibitors
 Piperazines
 Rats
 Rats, Sprague-Dawley
 Sulfones
 Triazines
 Vardenafil Dihydrochloride
 Vasoconstrictor Agents
 Vasodilation
 Vasodilator Agents
K10plus-PPN:1782003231
Verknüpfungen:→ Zeitschrift

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