| Online-Ressource |
Verfasst von: | Radovits, Tamás [VerfasserIn]  |
| Arif, Rawa [VerfasserIn]  |
| Bömicke, Timo [VerfasserIn]  |
| Korkmaz-İçöz, Sevil [VerfasserIn]  |
| Barnucz, Enikő [VerfasserIn]  |
| Karck, Matthias [VerfasserIn]  |
| Merkely, Béla [VerfasserIn]  |
| Szabó, Gábor [VerfasserIn]  |
Titel: | Vascular dysfunction induced by hypochlorite is improved by the selective phosphodiesterase-5-inhibitor vardenafil |
Verf.angabe: | Tamás Radovits, Rawa Arif, Timo Bömicke, Sevil Korkmaz, Enikő Barnucz, Matthias Karck, Béla Merkely, Gábor Szabó |
E-Jahr: | 2013 |
Jahr: | 23 April 2013 |
Umfang: | 10 S. |
Fussnoten: | Gesehen am 13.12.2021 |
Titel Quelle: | Enthalten in: European journal of pharmacology |
Ort Quelle: | New York, NY [u.a.] : Elsevier, 1967 |
Jahr Quelle: | 2013 |
Band/Heft Quelle: | 710(2013), 1/3 vom: 15. Juni, Seite 110-119 |
ISSN Quelle: | 1879-0712 |
Abstract: | Reactive oxygen species, such as hypochlorite induce oxidative stress, which impairs nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) signalling and leads to vascular dysfunction. It has been proposed, that elevated cGMP-levels may contribute to an effective cytoprotection against oxidative stress. We investigated the effects of vardenafil, a selective inhibitor of the cGMP-degrading phosphodiesterase-5 enzyme on vascular dysfunction induced by hypochlorite. In organ bath experiments for isometric tension, we investigated the endothelium-dependent and endothelium-independent vasorelaxation of isolated rat aortic rings using cumulative concentrations of acetylcholine and sodium nitroprusside (SNP). Vascular dysfunction was induced by exposing rings to hypochlorite (100-400 µM). In the treatment groups, rats were pretreated with vardenafil (30 and 300 µg/kg i.v.). Immunohistochemical analysis was performed for the oxidative stress markers nitrotyrosine, poly(ADP-ribose) and for apoptosis inducing factor (AIF). Exposure to hypochlorite resulted in a marked impairment of acetylcholine-induced endothelium-dependent vasorelaxation of aortic rings. Pretreatment with vardenafil led to improved endothelial function as reflected by the higher maximal vasorelaxation (Rmax) to acetylcholine. Regarding endothelium-independent vasorelaxation, hypochlorite exposure led to a left-shift of SNP concentration-response curves in the vardenafil groups without any alterations of the Rmax. In the hypochlorite groups immunohistochemical analysis showed enhanced poly(ADP-ribose)-formation and nuclear translocation of AIF, which were prevented by vardenafil-pretreatment. Our results support the view that cytoprotective effects of PDE-5-inhibitors on the endothelium may underlie the improved endothelial function, however, a slight sensitisation of vascular smooth muscle to NO was also confirmed. PDE-5-inhibition may represent a potential therapy approach for treating vascular dysfunction induced by oxidative stress. |
DOI: | doi:10.1016/j.ejphar.2013.04.012 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.ejphar.2013.04.012 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S0014299913003051?via%3Dihub |
| DOI: https://doi.org/10.1016/j.ejphar.2013.04.012 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Acetylcholine |
| Animals |
| Aorta, Thoracic |
| Endothelium, Vascular |
| Hypochlorous Acid |
| Imidazoles |
| In Vitro Techniques |
| Male |
| Nitroprusside |
| Oxidants |
| Oxidative Stress |
| Phenylephrine |
| Phosphodiesterase 5 Inhibitors |
| Piperazines |
| Rats |
| Rats, Sprague-Dawley |
| Sulfones |
| Triazines |
| Vardenafil Dihydrochloride |
| Vasoconstrictor Agents |
| Vasodilation |
| Vasodilator Agents |
K10plus-PPN: | 1782003231 |
Verknüpfungen: | → Zeitschrift |
Vascular dysfunction induced by hypochlorite is improved by the selective phosphodiesterase-5-inhibitor vardenafil / Radovits, Tamás [VerfasserIn]; 23 April 2013 (Online-Ressource)