| |  Online-Ressource |
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| Verfasst von: | Rühle, Alexander [VerfasserIn]  |
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| | Dai, Ping [VerfasserIn] |
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| | Lopez Perez, Ramon [VerfasserIn] |
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| | Strack, Maren [VerfasserIn] |
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| | Brons, Stephan [VerfasserIn] |
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| | Yijia, Qin [VerfasserIn] |
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| | Debus, Jürgen [VerfasserIn] |
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| | Wuchter, Patrick [VerfasserIn] |
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| | Grosu, Anca-Ligia [VerfasserIn] |
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| | Huber, Peter E. [VerfasserIn] |
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| | Nicolay, Nils [VerfasserIn] |
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| Titel: | Human mesenchymal stromal cells maintain their stem cell traits after high-LET particle irradiation - Potential implications for particle radiotherapy and manned space missions |
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| Verf.angabe: | Alexander Rühle, Dai Ping, Ramon Lopez Perez, Maren Strack, Stephan Brons, Qin Yijia, Jürgen Debus, Patrick Wuchter, Anca-Ligia Grosu, Peter E. Huber, Nils H. Nicolay |
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| Jahr: | 2022 |
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| Umfang: | 10 S. |
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| Fussnoten: | Available online 21 October 2021 ; Gesehen am 13.12.2021 |
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| Titel Quelle: | Enthalten in: Cancer letters |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1975 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 524(2022), Seite 172-181 |
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| ISSN Quelle: | 1872-7980 |
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| Abstract: | The influence of high-linear energy transfer (LET) particle radiation on the functionalities of mesenchymal stromal cells (MSCs) is largely unknown. Here, we analyzed the effects of proton (1H), helium (4He), carbon (12C) and oxygen (16O) ions on human bone marrow-MSCs. Cell cycle distribution and apoptosis induction were examined by flow cytometry, and DNA damage was quantified using γH2AX immunofluorescence and Western blots. Relative biological effectiveness values of MSCs amounted to 1.0-1.1 for 1H, 1.7-2.3 for 4He, 2.9-3.4 for 12C and 2.6-3.3 for 16O. Particle radiation did not alter the MSCs’ characteristic surface marker pattern, and MSCs maintained their multi-lineage differentiation capabilities. Apoptosis rates ranged low for all radiation modalities. At 24 h after irradiation, particle radiation-induced ATM and CHK2 phosphorylation as well as γH2AX foci numbers returned to baseline levels. The resistance of human MSCs to high-LET irradiation suggests that MSCs remain functional after exposure to moderate doses of particle radiation as seen in normal tissues after particle radiotherapy or during manned space flights. In the future, in vivo models focusing on long-term consequences of particle irradiation on the bone marrow niche and MSCs are needed. |
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| DOI: | doi:10.1016/j.canlet.2021.10.015 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.canlet.2021.10.015 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0304383521005255 |
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| | DOI: https://doi.org/10.1016/j.canlet.2021.10.015 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Carbon ions |
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| | Helium ions |
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| | High-LET radiation |
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| | Mesenchymal stem cells |
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| | Oxygen ions |
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| | Particle radiotherapy |
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| | Proton radiotherapy |
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| | Radiotherapy |
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| | Space radiation |
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| K10plus-PPN: | 1782015345 |
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| Verknüpfungen: | → Zeitschrift |
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Human mesenchymal stromal cells maintain their stem cell traits after high-LET particle irradiation - Potential implications for particle radiotherapy and manned space missions / Rühle, Alexander [VerfasserIn]; 2022 (Online-Ressource)
