Establishment of tumor treating fields combined with mild hyperthermia as novel supporting therapy for pancreatic cancer

• Verfasst von: Bai, Liping [VerfasserIn]
• Verfasst von: Pfeifer, Tobias [VerfasserIn]
• Verfasst von: Gross, Wolfgang [VerfasserIn]
• Verfasst von: Torre, Carolina de la [VerfasserIn]
• Verfasst von: Zhao, Shuyang [VerfasserIn]
• Verfasst von: Liu, Li [VerfasserIn]
• Verfasst von: Schäfer, Michael [VerfasserIn]
• Verfasst von: Herr, Ingrid [VerfasserIn]
• Titel: Establishment of tumor treating fields combined with mild hyperthermia as novel supporting therapy for pancreatic cancer
• Verf.angabe: Liping Bai, Tobias Pfeifer, Wolfgang Gross, Carolina De La Torre, Shuyang Zhao, Li Liu, Michael Schaefer and Ingrid Herr
• E-Jahr: 2021
• Jahr: 03 November 2021
• Umfang: 17 S.
• Fussnoten: Gesehen am 15.12.2021
• Titel Quelle: Enthalten in: Frontiers in oncology
• Ort Quelle: Lausanne : Frontiers Media, 2011
• Jahr Quelle: 2021
• Band/Heft Quelle: 11(2021) vom: 3. Nov., Artikel-ID 738801, Seite 1-17
• ISSN Quelle: 2234-943X
• DOI: doi:10.3389/fonc.2021.738801
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Errata: Bai, Liping, 1977 - : Corrigendum
• Bibliogr. Hinweis: Errata: Bai, Liping, 1977 - : Corrigendum: Establishment of tumor treating fields combined with mild hyperthermia as novel supporting therapy for pancreatic cancer
• K10plus-PPN: 1782251626
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a highly malignant tumor with poor prognosis and limited therapeutic options. Alternating electrical fields with low intensity called "Tumor Treating Fields" (TTFields) are a new, non-invasive approach with almost no side effects and phase 3 trials are ongoing in advanced PDAC. We evaluated TTFields in combination with mild hyperthermia. Three established human PDAC cell lines and an immortalized pancreatic duct cell line were treated with TTFields and hyperthermia at 38.5°C, followed by microscopy, assays for MTT, migration, colony and sphere formation, RT-qPCR, FACS, Western blot, microarray and bioinformatics, and in silico analysis using the online databases GSEA, KEGG, Cytoscape-String, and Kaplan-Meier Plotter. Whereas TTFields and hyperthermia alone had weak effects, their combination strongly inhibited the viability of malignant, but not those of nonmalignant cells. Progression features and the cell cycle were impaired, and autophagy was induced. The identified target genes were key players in autophagy, the cell cycle and DNA repair. The expression profiles of part of these target genes were significantly involved in the survival of PDAC patients. In conclusion, the combination of TTFields with mild hyperthermia results in greater efficacy without increased toxicity and could be easily clinically approved as supporting therapy.