RNAi suppressor P19 can be broadly exploited for enhanced adenovirus replication and microRNA knockdown experiments

• Verfasst von: Rauschhuber, Christina Theresa [VerfasserIn]
• Verfasst von: Mueck-Haeusl, Martin [VerfasserIn]
• Verfasst von: Zhang, Wenli [VerfasserIn]
• Verfasst von: Nettelbeck, Dirk M. [VerfasserIn]
• Verfasst von: Ehrhardt, Anja [VerfasserIn]
• Titel: RNAi suppressor P19 can be broadly exploited for enhanced adenovirus replication and microRNA knockdown experiments
• Verf.angabe: Christina Rauschhuber, Martin Mueck-Haeusl, Wenli Zhang, Dirk M. Nettelbeck & Anja Ehrhardt
• E-Jahr: 2013
• Jahr: 4 March 2013
• Umfang: 7 S.
• Fussnoten: Gesehen am 16.12.2021
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2013
• Band/Heft Quelle: 3(2013), 1, Artikel-ID 1363, Seite 1-7
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/srep01363
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Biotechnology
• Sach-SW: Gene delivery
• Sach-SW: Medical research
• Sach-SW: Molecular biology
• K10plus-PPN: 1782356533

Abstract

RNA interference (RNAi) is a key regulator of various biological systems including viral infection. Within a virus life cycle gene products can be modulated by the RNA interference (RNAi) pathway which can crucially impact productive virus replication. Herein we explored the RNA interference suppressor protein P19 derived from a plant virus and we found that P19 enhanced adenovirus replication up to 100-fold. Critical factors responsible for this observation were overexpression of adenovirus encoded genes on mRNA and protein levels. To investigate the impact of this phenomenon on recombinant viruses, we exploited its feasibility for therapeutic and genomic applications. We found that P19 significantly increased recombinant adenovirus yields enabling up-scaling for preclinical and clinical studies. Moreover, adenoviruses possessed significantly higher oncolytic activity by expression of P19. Finally, we show that introducing a p19 expression cassette into high-capacity adenovirus provides a strategy to analyze RNAi knockdown in a tissue-specific manner.