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Status: Bibliographieeintrag

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Verfasst von:Rechenmacher, Florian [VerfasserIn]   i
 Neubauer, Stefanie [VerfasserIn]   i
 Mas-Moruno, Carlos [VerfasserIn]   i
 Dorfner, Petra M. [VerfasserIn]   i
 Polleux, Julien [VerfasserIn]   i
 Guasch, Judith [VerfasserIn]   i
 Conings, Bert [VerfasserIn]   i
 Boyen, Hans-Gerd [VerfasserIn]   i
 Bochen, Alexander [VerfasserIn]   i
 Sobahi, Tariq R. [VerfasserIn]   i
 Burgkart, Rainer [VerfasserIn]   i
 Spatz, Joachim P. [VerfasserIn]   i
 Fässler, Reinhard [VerfasserIn]   i
 Kessler, Horst [VerfasserIn]   i
Titel:A molecular toolkit for the functionalization of titanium-based biomaterials that selectively control integrin-mediated cell adhesion
Verf.angabe:Florian Rechenmacher, Stefanie Neubauer, Carlos Mas-Moruno, Petra M. Dorfner, Julien Polleux, Judith Guasch, Bert Conings, Hans-Gerd Boyen, Alexander Bochen, Tariq R. Sobahi, Rainer Burgkart, Joachim P. Spatz, Reinhard Fässler, and Horst Kessler
E-Jahr:2013
Jahr:June 6, 2013
Umfang:6 S.
Fussnoten:Gesehen am 17.12.2021
Titel Quelle:Enthalten in: Chemistry - a European journal
Ort Quelle:Weinheim : Wiley-VCH, 1995
Jahr Quelle:2013
Band/Heft Quelle:19(2013), 28, Seite 9218-9223
ISSN Quelle:1521-3765
Abstract:We present a click chemistry-based molecular toolkit for the biofunctionalization of materials to selectively control integrin-mediated cell adhesion. To this end, α5β1-selective RGD peptidomimetics were covalently immobilized on Ti-based materials, and the capacity to promote the selective binding of α5β1 was evaluated using a solid-phase integrin binding assay. This functionalization strategy yielded surfaces with a nine-fold increased affinity for α5β1, in comparison to control samples, and total selectivity against the binding of the closely related integrin αvβ3. Moreover, our methodology allowed the screening of several phosphonic acid containing anchoring units to find the best spacer-anchor moiety required for establishing an efficient binding to titanium and to promote selective integrin binding. The integrin subtype specificity of these biofunctionalized surfaces was further examined in vitro by inducing selective adhesion of genetically modified fibroblasts, which express exclusively the α5β1 integrin. The versatility of our molecular toolkit was proven by shifting the cellular specificity of the materials from α5β1- to αvβ3-expressing fibroblasts by using an αvβ3-selective peptidomimetic as coating molecule. The results shown here represent the first functionalization of Ti-based materials with α5β1- or αvβ3-selective peptidomimetics that allow an unprecedented control to discriminate between α5β1- and αvβ3-mediated adhesions. The role of these two integrins in different biological events is still a matter of debate and is frequently discussed in literature. Thus, such bioactive titanium surfaces will be of great relevance for the study of integrin-mediated cell adhesion and the development of new biomaterials targeting specific cell types.
DOI:doi:10.1002/chem.201301478
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1002/chem.201301478
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201301478
 DOI: https://doi.org/10.1002/chem.201301478
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:biomaterials
 cell adhesion
 click chemistry
 integrins
 peptidomimetics
 surface coating
 titanium
K10plus-PPN:1782409777
Verknüpfungen:→ Zeitschrift

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