| |  Online-Ressource |
|---|
| Verfasst von: | Rastgou Talemi, Soheil [VerfasserIn]  |
|---|
| | Bartenschlager, Marie [VerfasserIn] |
|---|
| | Schmid, Bianca [VerfasserIn] |
|---|
| | Ruggieri, Alessia [VerfasserIn] |
|---|
| | Bartenschlager, Ralf [VerfasserIn] |
|---|
| | Höfer, Thomas [VerfasserIn] |
|---|
| Titel: | Dengue virus is sensitive to inhibition prior to productive replication |
|---|
| Verf.angabe: | Soheil Rastgou Talemi, Marie Bartenschlager, Bianca Schmid, Alessia Ruggieri, Ralf Bartenschlager, and Thomas Höfer |
|---|
| E-Jahr: | 2021 |
|---|
| Jahr: | 12 October 2021 |
|---|
| Umfang: | 13 S. |
|---|
| Fussnoten: | Gesehen am 20.12.2021 |
|---|
| Titel Quelle: | Enthalten in: Cell reports |
|---|
| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
|---|
| Jahr Quelle: | 2021 |
|---|
| Band/Heft Quelle: | 37(2021), 2, Artikel-ID 109801, Seite 1-13 |
|---|
| ISSN Quelle: | 2211-1247 |
|---|
| Abstract: | Uncovering vulnerable steps in the life cycle of viruses supports the rational design of antiviral treatments. However, information on viral replication dynamics obtained from traditional bulk assays with host cell populations is inherently limited as the data represent averages over a multitude of unsynchronized replication cycles. Here, we use time-lapse imaging of virus replication in thousands of single cells, combined with computational inference, to identify rate-limiting steps for dengue virus (DENV), a widespread human pathogen. Comparing wild-type DENV with a vaccine candidate mutant, we show that the viral spread in the mutant is greatly attenuated by delayed onset of productive replication, whereas wild-type and mutant virus have identical replication rates. Single-cell analysis done after applying the broad-spectrum antiviral drug, ribavirin, at clinically relevant concentrations revealed the same mechanism of attenuating viral spread. We conclude that the initial steps of infection, rather than the rate of established replication, are quantitatively limiting DENV spread. |
|---|
| DOI: | doi:10.1016/j.celrep.2021.109801 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.celrep.2021.109801 |
|---|
| | Volltext: https://www.sciencedirect.com/science/article/pii/S2211124721012614 |
|---|
| | DOI: https://doi.org/10.1016/j.celrep.2021.109801 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | 2'-O methylation |
|---|
| | dengue virus |
|---|
| | IFIT1 |
|---|
| | innate immune response |
|---|
| | interferons |
|---|
| | mathematical modeling |
|---|
| | MX1 |
|---|
| | quantitative single-cell analysis |
|---|
| | ribavirin |
|---|
| | RNA virus replication |
|---|
| K10plus-PPN: | 1782623019 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Dengue virus is sensitive to inhibition prior to productive replication / Rastgou Talemi, Soheil [VerfasserIn]; 12 October 2021 (Online-Ressource)
