| |  Online-Ressource |
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| Verfasst von: | Twu, Woan-Ing [VerfasserIn]  |
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| | Lee, Ji Young [VerfasserIn] |
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| | Kim, Heeyoung [VerfasserIn] |
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| | Prasad, Vibhu [VerfasserIn] |
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| | Cerikan, Berati [VerfasserIn] |
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| | Haselmann, Uta [VerfasserIn] |
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| | Tabata, Keisuke [VerfasserIn] |
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| | Bartenschlager, Ralf [VerfasserIn] |
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| Titel: | Contribution of autophagy machinery factors to HCV and SARS-CoV-2 replication organelle formation |
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| Verf.angabe: | Woan-Ing Twu, Ji-Young Lee, Heeyoung Kim, Vibhu Prasad, Berati Cerikan, Uta Haselmann, Keisuke Tabata, Ralf Bartenschlager |
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| E-Jahr: | 2021 |
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| Jahr: | 10 November 2021 |
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| Umfang: | 22 S. |
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| Fussnoten: | Gesehen am 07.01.2022 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 37(2021), 8, Artikel-ID 110049, Seite 1-22 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | Positive-strand RNA viruses replicate in close association with rearranged intracellular membranes. For hepatitis C virus (HCV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), these rearrangements comprise endoplasmic reticulum (ER)-derived double membrane vesicles (DMVs) serving as RNA replication sites. Cellular factors involved in DMV biogenesis are poorly defined. Here, we show that despite structural similarity of viral DMVs with autophagosomes, conventional macroautophagy is dispensable for HCV and SARS-CoV-2 replication. However, both viruses exploit factors involved in autophagosome formation, most notably class III phosphatidylinositol 3-kinase (PI3K). As revealed with a biosensor, PI3K is activated in cells infected with either virus to produce phosphatidylinositol 3-phosphate (PI3P) while kinase complex inhibition or depletion profoundly reduces replication and viral DMV formation. The PI3P-binding protein DFCP1, recruited to omegasomes in early steps of autophagosome formation, participates in replication and DMV formation of both viruses. These results indicate that phylogenetically unrelated HCV and SARS-CoV-2 exploit similar components of the autophagy machinery to create their replication organelles. |
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| DOI: | doi:10.1016/j.celrep.2021.110049 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.celrep.2021.110049 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S2211124721015357 |
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| | DOI: https://doi.org/10.1016/j.celrep.2021.110049 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | autophagy |
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| | Beclin 1 |
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| | class III PI3K |
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| | coronavirus |
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| | daclatasvir |
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| | DFCP1 |
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| | DMV |
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| | hepatitis C virus |
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| | PI3P |
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| | replication organelle |
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| K10plus-PPN: | 1785215027 |
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| Verknüpfungen: | → Zeitschrift |
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Contribution of autophagy machinery factors to HCV and SARS-CoV-2 replication organelle formation / Twu, Woan-Ing [VerfasserIn]; 10 November 2021 (Online-Ressource)
