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| Verfasst von: | García-García, Laura [VerfasserIn]  |
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| | Fernández-Tabanera, Enrique [VerfasserIn] |
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| | Cervera, Saint T. [VerfasserIn] |
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| | Melero-Fernández de Mera, Raquel M. [VerfasserIn] |
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| | Josa, Santiago [VerfasserIn] |
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| | González-González, Laura [VerfasserIn] |
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| | Rodríguez-Martín, Carlos [VerfasserIn] |
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| | Grünewald, Thomas G. P. [VerfasserIn] |
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| | Alonso, Javier [VerfasserIn] |
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| Titel: | The transcription factor FEZF1, a direct target of EWSR1-FLI1 in Ewing sarcoma cells, regulates the expression of neural-specific genes |
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| Verf.angabe: | Laura García-García, Enrique Fernández-Tabanera, Saint T. Cervera, Raquel M. Melero-Fernández de Mera, Santiago Josa, Laura González-González, Carlos Rodríguez-Martín, Thomas G.P. Grünewald and Javier Alonso |
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| E-Jahr: | 2021 |
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| Jahr: | 12 November 2021 |
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| Umfang: | 18 S. |
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| Fussnoten: | Gesehen am 07.01.2022 |
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| Titel Quelle: | Enthalten in: Cancers |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 13(2021), 22, Artikel-ID 5668, Seite 1-18 |
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| ISSN Quelle: | 2072-6694 |
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| Abstract: | Ewing sarcoma is a rare pediatric tumor characterized by chromosomal translocations that give rise to aberrant chimeric transcription factors (e.g., EWSR1-FLI1). EWSR1-FLI1 promotes a specific cellular transcriptional program. Therefore, the study of EWSR1-FLI1 target genes is important to identify critical pathways involved in Ewing sarcoma tumorigenesis. In this work, we focused on the transcription factors regulated by EWSR1-FLI1 in Ewing sarcoma. Transcriptomic analysis of the Ewing sarcoma cell line A673 indicated that one of the genes more strongly upregulated by EWSR1-FLI1 was FEZF1 (FEZ family zinc finger protein 1), a transcriptional repressor involved in neural cell identity. The functional characterization of FEZF1 was performed in three Ewing sarcoma cell lines (A673, SK-N-MC, SK-ES-1) through an shRNA-directed silencing approach. FEZF1 knockdown inhibited clonogenicity and cell proliferation. Finally, the analysis of the FEZF1-dependent expression profile in A673 cells showed several neural genes regulated by FEZF1 and concomitantly regulated by EWSR1-FLI1. In summary, FEZF1 is transcriptionally regulated by EWSR1-FLI1 in Ewing sarcoma cells and is involved in the regulation of neural-specific genes, which could explain the neural-like phenotype observed in several Ewing sarcoma tumors and cell lines. |
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| DOI: | doi:10.3390/cancers13225668 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/cancers13225668 |
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| | Volltext: https://www.mdpi.com/2072-6694/13/22/5668 |
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| | DOI: https://doi.org/10.3390/cancers13225668 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Ewing sarcoma |
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| | EWSR1-FLI1 |
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| | FEZF11 |
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| | GGAA-microsatellites |
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| K10plus-PPN: | 1785219413 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ transcription factor FEZF1, a direct target of EWSR1-FLI1 in Ewing sarcoma cells, regulates the expression of neural-specific genes / García-García, Laura [VerfasserIn]; 12 November 2021 (Online-Ressource)
