The FBMD-1 stroma cell line secretes a unique moiety which can increase retroviral transduction of lineage-committed and primitive human peripheral blood progenitor cells

• Verfasst von: Buß, Eike Christian [VerfasserIn]
• Verfasst von: Schiedlmeier, Bernhard [VerfasserIn]
• Verfasst von: Ho, Anthony Dick [VerfasserIn]
• Verfasst von: Zeller, W. Jens [VerfasserIn]
• Verfasst von: Frühauf, Stefan [VerfasserIn]
• Titel: The FBMD-1 stroma cell line secretes a unique moiety which can increase retroviral transduction of lineage-committed and primitive human peripheral blood progenitor cells
• Verf.angabe: Eike C. Buss, Bernd Schiedlmeier, Anthony D. Ho, W. Jens Zeller, and Stefan Fruehauf
• E-Jahr: 2001
• Jahr: 09 August 2001
• Umfang: 10 S.
• Fussnoten: Gesehen am 13.01.2022
• Titel Quelle: Enthalten in: Cancer gene therapy
• Ort Quelle: New York, NY : Nature Publ. Group, 1999
• Jahr Quelle: 2001
• Band/Heft Quelle: 8(2001), 6, Seite 440-449
• ISSN Quelle: 1476-5500
• DOI: doi:10.1038/sj.cgt.7700324
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1786006537

Abstract

Peripheral blood progenitor cells are a prime target for gene therapy approaches. As recent data point to the relevance of soluble stroma factors for the efficient transduction of progenitor cells, we tested the stroma-conditioned medium (SCM) of the two cell lines FBMD-1 and L88/5 as well as desulfated and O-sulfated heparin (HS dS and HS OS) for their effect on transduction of peripheral blood progenitor cells. We transduced CD34 + cells of nine tumor patients with the retroviral SF-MDR vector containing the human multidrug resistance 1 ( MDR 1) gene under serum-free conditions on the fibronectin fragment CH-296 with or without SCM.