Gene expression changes in vastus lateralis muscle after different strength training regimes during rehabilitation following anterior cruciate ligament reconstruction

• Verfasst von: Friedmann-Bette, Birgit [VerfasserIn]
• Verfasst von: Lornsen, Holger [VerfasserIn]
• Verfasst von: Parstorfer, Mario [VerfasserIn]
• Verfasst von: Gwechenberger, Thomas [VerfasserIn]
• Verfasst von: Profit, Francesca [VerfasserIn]
• Verfasst von: Weber, Marc-André [VerfasserIn]
• Verfasst von: Barié, Alexander [VerfasserIn]
• Titel: Gene expression changes in vastus lateralis muscle after different strength training regimes during rehabilitation following anterior cruciate ligament reconstruction
• Verf.angabe: Birgit Friedmann-Bette, Holger Lornsen, Mario Parstorfer, Thomas Gwechenberger, Francesca Profit, Marc-André Weber, Alexander Barié
• E-Jahr: 2021
• Jahr: October 14, 2021
• Umfang: 18 S.
• Fussnoten: Gesehen am 20.01.2021
• Titel Quelle: Enthalten in: PLOS ONE
• Ort Quelle: San Francisco, California, US : PLOS, 2006
• Jahr Quelle: 2021
• Band/Heft Quelle: 16(2021), 10, Artikel-ID e0258635, Seite 1-18
• ISSN Quelle: 1932-6203
• DOI: doi:10.1371/journal.pone.0258635
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Atrophy
• Sach-SW: Autocrine signaling
• Sach-SW: Biopsy
• Sach-SW: Gene expression
• Sach-SW: Metabolic syndrome
• Sach-SW: Myotonic dystrophy
• Sach-SW: Skeletal muscles
• Sach-SW: Strength training
• K10plus-PPN: 1786604043

Abstract

Impaired muscle regeneration has repeatedly been described after anterior cruciate ligament reconstruction (ACL-R). The results of recent studies provided some evidence for negative alterations in knee extensor muscles after ACL-R causing persisting strength deficits in spite of the regain of muscle mass. Accordingly, we observed that 12 weeks of concentric/eccentric quadriceps strength training with eccentric overload (CON/ECC+) induced a significantly greater hypertrophy of the atrophied quadriceps muscle after ACL-R than conventional concentric/eccentric quadriceps strength training (CON/ECC). However, strength deficits persisted and there was an unexpected increase in the proportion of slow type I fibers instead of the expected shift towards a faster muscle phenotype after CON/ECC+. In order to shed further light on muscle recovery after ACL-R, the steady-state levels of 84 marker mRNAs were analyzed in biopsies obtained from the vastus lateralis muscle of 31 subjects before and after 12 weeks of CON/ECC+ (n = 18) or CON/ECC strength training (n = 13) during rehabilitation after ACL-R using a custom RT2 Profiler PCR array. Significant (p < 0.05) changes were detected in the expression of 26 mRNAs, several of them involved in muscle wasting/atrophy. A different pattern with regard to the strength training mode was observed for 16 mRNAs, indicating an enhanced hypertrophic stimulus, mechanical sensing or fast contractility after CON/ECC+. The effects of the type of autograft (quadriceps, QUAD, n = 19, or semitendinosus tendon, SEMI, n = 12) were reflected in the lower expression of 6 mRNAs involved in skeletal muscle hypertrophy or contractility in QUAD. In conclusion, the greater hypertrophic stimulus and mechanical stress induced by CON/ECC+ and a beginning shift towards a faster muscle phenotype after CON/ECC+ might be indicated by significant gene expression changes as well as still ongoing muscle wasting processes and a negative impact of QUAD autograft.