| |  Online-Ressource |
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| Verfasst von: | Sherwood, Anna [VerfasserIn]  |
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| | Emerson, Ryan O. [VerfasserIn] |
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| | Scherer, Dominique [VerfasserIn] |
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| | Habermann, Nina [VerfasserIn] |
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| | Buck, Katharina [VerfasserIn] |
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| | Staffa, Jürgen [VerfasserIn] |
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| | Desmarais, Cindy [VerfasserIn] |
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| | Halama, Niels [VerfasserIn] |
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| | Jäger, Dirk [VerfasserIn] |
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| | Schirmacher, Peter [VerfasserIn] |
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| | Herpel, Esther [VerfasserIn] |
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| | Kloor, Matthias [VerfasserIn] |
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| | Ulrich, Alexis [VerfasserIn] |
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| | Schneider, Martin [VerfasserIn] |
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| | Ulrich, Cornelia [VerfasserIn] |
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| | Robins, Harlan [VerfasserIn] |
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| Titel: | Tumor-infiltrating lymphocytes in colorectal tumors display a diversity of T cell receptor sequences that differ from the T cells in adjacent mucosal tissue |
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| Verf.angabe: | Anna M. Sherwood, Ryan O. Emerson, Dominique Scherer, Nina Habermann, Katharina Buck, Jürgen Staffa, Cindy Desmarais, Niels Halama, Dirk Jaeger, Peter Schirmacher, Esther Herpel, Matthias Kloor, Alexis Ulrich, Martin Schneider, Cornelia M. Ulrich, Harlan Robins |
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| Jahr: | 2013 |
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| Umfang: | 9 S. |
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| Fussnoten: | Published online: 16 June 2013 ; Gesehen am 25.01.2022 |
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| Titel Quelle: | Enthalten in: Cancer immunology immunotherapy |
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| Ort Quelle: | Berlin : Springer, 1976 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 62(2013), 9, Seite 1453-1461 |
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| ISSN Quelle: | 1432-0851 |
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| Abstract: | Tumors from colorectal cancer (CRC) are generally immunogenic and commonly infiltrated with T lymphocytes. However, the details of the adaptive immune reaction to these tumors are poorly understood. We have accrued both colon tumor samples and adjacent healthy mucosal samples from 15 CRC patients to study lymphocytes infiltrating these tissues. We apply a method for detailed sequencing of T-cell receptor (TCR) sequences from tumor-infiltrating lymphocytes (TILs) in CRC tumors at high throughput to probe T-cell clones in comparison with the TCRs from adjacent healthy mucosal tissue. In parallel, we captured TIL counts using standard immunohistochemistry. The variation in diversity of the TIL repertoire was far wider than the variation of T-cell clones in the healthy mucosa, and the oligoclonality was higher on average in the tumors. However, the diversity of the T-cell repertoire in both CRC tumors and healthy mucosa was on average 100-fold lower than in peripheral blood. Using the TCR sequences to identify and track clones between mucosal and tumor samples, we determined that the immune response in the tumor is different than in the adjacent mucosal tissue, and the number of shared clones is not dependent on distance between the samples. Together, these data imply that CRC tumors induce a specific adaptive immune response, but that this response differs widely in strength and breadth between patients. |
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| DOI: | doi:10.1007/s00262-013-1446-2 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1007/s00262-013-1446-2 |
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| | Volltext: https://link.springer.com/article/10.1007/s00262-013-1446-2 |
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| | DOI: https://doi.org/10.1007/s00262-013-1446-2 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 178708440X |
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| Verknüpfungen: | → Zeitschrift |
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Tumor-infiltrating lymphocytes in colorectal tumors display a diversity of T cell receptor sequences that differ from the T cells in adjacent mucosal tissue / Sherwood, Anna [VerfasserIn]; 2013 (Online-Ressource)
