Microvascular changes following four-hour single arteriole occlusion

• Verfasst von: Saltzman, Darin James [VerfasserIn]
• Verfasst von: Kerger, Heinz [VerfasserIn]
• Verfasst von: Jimenez, Juan Carlos [VerfasserIn]
• Verfasst von: Farzan, Dina [VerfasserIn]
• Verfasst von: Wilson, James M. [VerfasserIn]
• Verfasst von: Thompson, Jesse E. [VerfasserIn]
• Verfasst von: Intaglietta, Marcos [VerfasserIn]
• Titel: Microvascular changes following four-hour single arteriole occlusion
• Verf.angabe: Darin J. Saltzman, MD, PhD, Heinz Kerger, MD, Juan Carlos Jimenez, MD, FACZ, Dina Farzan, BSc, James M. Wilson, MD, Jesse E. Thompson, MD, and Marcos Intaglietta, PhD
• Jahr: 2013
• Umfang: 9 S.
• Fussnoten: Published online 14 November 2012 ; Gesehen am 27.01.2022
• Titel Quelle: Enthalten in: Microsurgery
• Ort Quelle: New York, NY [u.a.] : Wiley-Liss, 1979
• Jahr Quelle: 2013
• Band/Heft Quelle: 33(2013), 3 vom: März, Seite 207-215
• ISSN Quelle: 1098-2752
• DOI: doi:10.1002/micr.22051
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1787306224

Abstract

Background: Free tissue transplantations are lengthy procedures that result in prolong tissue ischemia. Restoral of blood flow is essential for free flap recovery; however, upon reperfusion tissue that is viable may continue to be nonperfused. To further elucidate this pathophysiology skeletal muscle microcirculation was investigated during reperfusion following 4-hour single arteriole occlusion. Materials and methods: A blunt micropipette probe was use to compress a single arteriole in the unanesthetized hamster (N = 20) dorsal skinfold chamber. Arteriole (n = 20), capillary (n = 97), and postcapillary venule (n = 16) diameters and blood flow were analyzed at 0, 30, 60, 120, 240 min and 24 hours of reperfusion after 4 hour occlusion. Results: Feeding arcade arterioles exhibited a brief (<10 min) vasoconstriction [0.31 ± 0.26 (mean ± SE) of baseline] upon reperfusion followed by a maximum vasodilation at 120 min (1.3 ± 0.10: P < 0.05). Vasodilation was observed in transverse arterioles (A3) (1.8 ± 0.20: P < 0.05). Correspondingly, all arteriole and venule flow was increased by 120 min (P < 0.05) of reperfusion. There was a transient decrease in the number of flowing capillaries at 0 and 30 min reperfusion (0.73 ± 0.09 and 0.84 ± 0.06: P < 0.05, respectively). Conclusions: At the onset of reperfusion heterogeneous arteriole flow and transient decrease in flowing capillaries was observed; however, return of flow in all capillaries and an eventual hyperemic response in all arterioles suggests the reversible nature of this response. Single arteriole occlusion may allow for a more controlled and detailed microcirculatory analysis during ischemia-reperfusion. © 2012 Wiley Periodicals, Inc. Microsurgery, 2013.