| |  Online-Ressource |
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| Verfasst von: | Szabó, Áron [VerfasserIn]  |
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| | Papin, Christian [VerfasserIn] |
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| | Zorn, Daniela [VerfasserIn] |
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| | Ponien, Prishila [VerfasserIn] |
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| | Weber, Frank [VerfasserIn] |
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| | Raabe, Thomas [VerfasserIn] |
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| | Rouyer, François [VerfasserIn] |
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| Titel: | The CK2 kinase stabilizes CLOCK and represses its activity in the Drosophila circadian oscillator |
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| Verf.angabe: | Áron Szabó, Christian Papin, Daniela Zorn, Prishila Ponien, Frank Weber, Thomas Raabe, François Rouyer |
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| E-Jahr: | 2013 |
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| Jahr: | August 27, 2013 |
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| Umfang: | 16 S. |
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| Fussnoten: | Gesehen am 27.01.2022 |
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| Titel Quelle: | Enthalten in: Public Library of SciencePLoS biology |
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| Ort Quelle: | Lawrence, KS : PLoS, 2003 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 11(2013), 8, Artikel-ID e1001645, Seite 1-16 |
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| ISSN Quelle: | 1545-7885 |
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| Abstract: | Phosphorylation is a pivotal regulatory mechanism for protein stability and activity in circadian clocks regardless of their evolutionary origin. It determines the speed and strength of molecular oscillations by acting on transcriptional activators and their repressors, which form negative feedback loops. In Drosophila, the CK2 kinase phosphorylates and destabilizes the PERIOD (PER) and TIMELESS (TIM) proteins, which inhibit CLOCK (CLK) transcriptional activity. Here we show that CK2 also targets the CLK activator directly. Downregulating the activity of the catalytic α subunit of CK2 induces CLK degradation, even in the absence of PER and TIM. Unexpectedly, the regulatory β subunit of the CK2 holoenzyme is not required for the regulation of CLK stability. In addition, downregulation of CK2α activity decreases CLK phosphorylation and increases per and tim transcription. These results indicate that CK2 inhibits CLK degradation while reducing its activity. Since the CK1 kinase promotes CLK degradation, we suggest that CLK stability and transcriptional activity result from counteracting effects of CK1 and CK2. |
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| DOI: | doi:10.1371/journal.pbio.1001645 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pbio.1001645 |
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| | Volltext: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001645 |
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| | DOI: https://doi.org/10.1371/journal.pbio.1001645 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Circadian oscillators |
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| | Drosophila melanogaster |
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| | Gene expression |
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| | Immunoprecipitation |
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| | Messenger RNA |
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| | Phosphorylation |
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| | Protein extraction |
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| | RNA interference |
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| K10plus-PPN: | 178732611X |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ CK2 kinase stabilizes CLOCK and represses its activity in the Drosophila circadian oscillator / Szabó, Áron [VerfasserIn]; August 27, 2013 (Online-Ressource)
