| Online-Ressource |
Verfasst von: | Shytaj, Iart Luca [VerfasserIn]  |
| Procopio, Francesco Andrea [VerfasserIn]  |
| Tarek, Mohammad [VerfasserIn]  |
| Carlon-Andres, Irene [VerfasserIn]  |
| Tang, Hsin-Yao [VerfasserIn]  |
| Goldman, Aaron R. [VerfasserIn]  |
| Munshi, MohamedHusen [VerfasserIn]  |
| Kumar Pal, Virender [VerfasserIn]  |
| Forcato, Mattia [VerfasserIn]  |
| Sreeram, Sheetal [VerfasserIn]  |
| Leskov, Konstantin [VerfasserIn]  |
| Ye, Fengchun [VerfasserIn]  |
| Lucic, Bojana [VerfasserIn]  |
| Cruz, Nicolly [VerfasserIn]  |
| Ndhlovu, Lishomwa C. [VerfasserIn]  |
| Bicciato, Silvio [VerfasserIn]  |
| Padilla-Parra, Sergi [VerfasserIn]  |
| Diaz, Ricardo Sobhie [VerfasserIn]  |
| Singh, Amit [VerfasserIn]  |
| Lusic, Marina [VerfasserIn]  |
| Karn, Jonathan [VerfasserIn]  |
| Alvarez-Carbonell, David [VerfasserIn]  |
| Savarino, Andrea [VerfasserIn]  |
Titel: | Glycolysis downregulation is a hallmark of HIV-1 latency and sensitizes infected cells to oxidative stress |
Verf.angabe: | Iart Luca Shytaj, Francesco Andrea Procopio, Mohammad Tarek, Irene Carlon-Andres, Hsin-Yao Tang, Aaron R. Goldman, MohamedHusen Munshi, Virender Kumar Pal, Mattia Forcato, Sheetal Sreeram, Konstantin Leskov, Fengchun Ye, Bojana Lucic, Nicolly Cruz, Lishomwa C. Ndhlovu, Silvio Bicciato, Sergi Padilla-Parra, Ricardo Sobhie Diaz, Amit Singh, Marina Lusic, Jonathan Karn, David Alvarez-Carbonell & Andrea Savarino |
E-Jahr: | 2021 |
Jahr: | 20 July 2021 |
Umfang: | 20 S. |
Fussnoten: | Gesehen am 27.01.2022 |
Titel Quelle: | Enthalten in: European Molecular Biology OrganizationEMBO molecular medicine |
Ort Quelle: | Weinheim : Wiley-VCH, 2009 |
Jahr Quelle: | 2021 |
Band/Heft Quelle: | 13(2021), 8, Artikel-ID e13901, Seite 1-20 |
ISSN Quelle: | 1757-4684 |
Abstract: | HIV-1 infects lymphoid and myeloid cells, which can harbor a latent proviral reservoir responsible for maintaining lifelong infection. Glycolytic metabolism has been identified as a determinant of susceptibility to HIV-1 infection, but its role in the development and maintenance of HIV-1 latency has not been elucidated. By combining transcriptomic, proteomic, and metabolomic analyses, we here show that transition to latent HIV-1 infection downregulates glycolysis, while viral reactivation by conventional stimuli reverts this effect. Decreased glycolytic output in latently infected cells is associated with downregulation of NAD+ /NADH. Consequently, infected cells rely on the parallel pentose phosphate pathway and its main product, NADPH, fueling antioxidant pathways maintaining HIV-1 latency. Of note, blocking NADPH downstream effectors, thioredoxin and glutathione, favors HIV-1 reactivation from latency in lymphoid and myeloid cellular models. This provides a "shock and kill effect" decreasing proviral DNA in cells from people living with HIV/AIDS. Overall, our data show that downmodulation of glycolysis is a metabolic signature of HIV-1 latency that can be exploited to target latently infected cells with eradication strategies. |
DOI: | doi:10.15252/emmm.202013901 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.15252/emmm.202013901 |
| DOI: https://doi.org/10.15252/emmm.202013901 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | CD4-Positive T-Lymphocytes |
| Down-Regulation |
| glycolysis |
| Glycolysis |
| HIV Infections |
| HIV-1 |
| HIV-1 latency |
| Humans |
| oxidative stress |
| Oxidative Stress |
| pentose cycle |
| Proteomics |
| pyrimidine metabolism |
| Virus Activation |
| Virus Latency |
K10plus-PPN: | 1787346668 |
Verknüpfungen: | → Zeitschrift |
Glycolysis downregulation is a hallmark of HIV-1 latency and sensitizes infected cells to oxidative stress / Shytaj, Iart Luca [VerfasserIn]; 20 July 2021 (Online-Ressource)