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Verfasst von:Gazyakan, Emre [VerfasserIn]   i
 Hirche, Christoph [VerfasserIn]   i
 Reichenberger, Matthias [VerfasserIn]   i
 Urbach, Olena [VerfasserIn]   i
 Germann, Günter [VerfasserIn]   i
 Engel, Holger [VerfasserIn]   i
Titel:Modulation of nitric oxide bioavailability attenuates ischemia-reperfusion injury in type II diabetes
Verf.angabe:Emre Gazyakan, Christoph Hirche, Matthias A. Reichenberger, Olena Urbach, Günter Germann, Holger Engel
E-Jahr:2021
Jahr:January 2021
Umfang:9 S.
Titel Quelle:Enthalten in: Journal of plastic, reconstructive & aesthetic surgery
Ort Quelle:Amsterdam [u.a.] : Elsevier, 2006
Jahr Quelle:2021
Band/Heft Quelle:74(2021), 1, Seite 183-191
ISSN Quelle:1878-0539
Abstract:Background - Diabetes mellitus increases the susceptibility of free tissue transplantations to ischemia-reperfusion injury. The aim of this study was to enhance nitric oxide (NO) bioavailability through exogenous NO synthase and the substrate L-arginine to attenuate ischemia reperfusion-induced alterations in a type 2 diabetes rodent model. - Material and Methods - Sixty-four Wistar rats were divided into 8 experimental groups. Type 2 diabetes was established over 3 months with a combination of a high-fat diet and streptozotocin. A vascular pedicle isolated rat skin flap model that underwent 3 h of ischemia was used. At 30 min before ischemia, normal saline, endothelial NOSs (eNOSs), inducible NOSs, neuronal NOSs (1 and 2 IU), and L-arginine (50 mg/kg body weight) were administered by intravenous infusion alone or in combination. Ischemia-reperfusion-induced alterations were measured 5 days after the operation. - Results - The three isoforms of NOS significantly increased the flap vitality rate (VR) between 20% and 28% as compared to the control group (3%). Sole L-arginine administration increased the VR to 33%. The combination of L-arginine with NOS resulted in a further increase in flap VRs (39%-50%). Best results were achieved with the combination of eNOS and L-arginine (50%). An increase in enzyme dosage led to decreased VRs in all NOS isoforms alone and even in combination with L-arginine. - Conclusion - Modulation of NO bioavailability through the exogenous application of NOSs and L-arginine significantly attenuated ischemia-reperfusion-induced alterations in a type 2 diabetic skin flap rat model. The combination of enzyme and substrate result in the highest VRs. Higher enzyme dosage seems to be less effective. This pharmacological preconditioning could be an easy and effective interventional strategy to support the conversion of L-arginine to NO in ischemic and in type 2 diabetic conditions.
DOI:doi:10.1016/j.bjps.2020.08.021
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.bjps.2020.08.021
 Volltext: https://www.sciencedirect.com/science/article/pii/S174868152030348X
 DOI: https://doi.org/10.1016/j.bjps.2020.08.021
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Bioavailability
 Ischemia-Reperfusion Injury
 L-arginine
 Microcirculation
 Nitric oxide
 Nitric oxide synthase
K10plus-PPN:1787821803
Verknüpfungen:→ Zeitschrift

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