Antioxidant and antiapoptotic effects of a Turraea fischeri leaf extract on cryopreserved goat sperm

• Verfasst von: Hassan, Soha Abdelaliem Hassan [VerfasserIn]
• Verfasst von: Khalil, Wael A. [VerfasserIn]
• Verfasst von: Hassan, Mahmoud A. E. [VerfasserIn]
• Verfasst von: Yousif, Ahmed I. [VerfasserIn]
• Verfasst von: Sabry, Omar M. [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Verfasst von: Sobeh, Mansour [VerfasserIn]
• Titel: Antioxidant and antiapoptotic effects of a Turraea fischeri leaf extract on cryopreserved goat sperm
• Verf.angabe: Soha A. Hassan, Wael A. Khalil, Mahmoud A.E. Hassan, Ahmed I. Yousif, Omar M. Sabry, Michael Wink and Mansour Sobeh
• E-Jahr: 2021
• Jahr: 29 September 2021
• Umfang: 13 S.
• Fussnoten: Gesehen am 04.02.2022
• Titel Quelle: Enthalten in: MDPIAnimals
• Ort Quelle: Basel : MDPI, 2011
• Jahr Quelle: 2021
• Band/Heft Quelle: 11(2021), 10, Artikel-ID 2840, Seite 1-13
• ISSN Quelle: 2076-2615
• DOI: doi:10.3390/ani11102840
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: <i>Turraea fischeri</i>
• Sach-SW: antioxidant biomarker
• Sach-SW: apoptosis
• Sach-SW: polyphenolics
• Sach-SW: semen cryopreservation
• Sach-SW: sperm ultrastructure
• K10plus-PPN: 1788503511

Abstract

This study evaluated the efficacy of Turraea fischeri leaf extract for maintaining the viability of cryopreserved goat sperm. Ejaculated semen was collected from 5 mature Baladi bucks (50-60 kg, 2-4 years of age) and those samples with mass motility ≥ 70% and sperm concentration ≥ 2.5 × 109/mL were selected, pooled, and divided into 4 aliquots. Each aliquot was diluted in Tris-citric-soybean lecithin extender containing a different concentration of T. fischeri leaf extract (0, 125, 250, or 375 µg/mL). Treated semen samples were cooled to 5 °C, transferred to 0.25-mL French straws, and stored in liquid nitrogen (LN2) at −196 °C. After thawing, membrane integrity was examined by transmission electron microscopy, apoptotic activity by Annexin/propidium iodide staining and flow cytometry, and both enzyme activities and antioxidant capacity by spectroscopic assays. The leaf extract at 375 µg/mL significantly improved semen quality as indicated by enhanced total antioxidant capacity, reduced H2O2 concentration, a greater proportion of structurally intact motile sperm, and concomitant reductions in apoptosis and necrosis. The extract also significantly increased the proportion of sperm with a contiguous plasma membrane and intact acrosome (p < 0.05). Furthermore, LC-MS revealed numerous secondary metabolites in the extract that may contribute to sperm cryopreservation.