| |  Online-Ressource |
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| Verfasst von: | Herzog, Rebecca [VerfasserIn]  |
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| | Bartosova, Maria [VerfasserIn] |
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| | Tarantino, Silvia [VerfasserIn] |
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| | Wagner, Anja [VerfasserIn] |
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| | Unterwurzacher, Markus [VerfasserIn] |
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| | Sacnun, Juan Manuel [VerfasserIn] |
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| | Lichtenauer, Anton M. [VerfasserIn] |
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| | Kuster, Lilian [VerfasserIn] |
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| | Schäfer, Betti [VerfasserIn] |
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| | Alper, Seth L. [VerfasserIn] |
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| | Aufricht, Christoph [VerfasserIn] |
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| | Schmitt, Claus P. [VerfasserIn] |
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| | Kratochwill, Klaus [VerfasserIn] |
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| Titel: | Peritoneal dialysis fluid supplementation with alanyl-glutamine attenuates conventional dialysis fluid-mediated endothelial cell injury by restoring perturbed cytoprotective responses |
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| Verf.angabe: | Rebecca Herzog, Maria Bartosova, Silvia Tarantino, Anja Wagner, Markus Unterwurzacher, Juan Manuel Sacnun, Anton M. Lichtenauer, Lilian Kuster, Betti Schaefer, Seth L. Alper, Christoph Aufricht, Claus Peter Schmit and Klaus Kratochwill |
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| E-Jahr: | 2020 |
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| Jahr: | 15 December 2020 |
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| Umfang: | 21 S. |
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| Fussnoten: | Gesehen am 09.02.2022 |
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| Titel Quelle: | Enthalten in: Biomolecules |
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| Ort Quelle: | Basel : MDPI, 2011 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 10(2020), 12, Artikel-ID 1678, Seite 1-21 |
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| ISSN Quelle: | 2218-273X |
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| Abstract: | Long-term clinical outcome of peritoneal dialysis (PD) depends on adequate removal of small solutes and water. The peritoneal endothelium represents the key barrier and peritoneal transport dysfunction is associated with vascular changes. Alanyl-glutamine (AlaGln) has been shown to counteract PD-induced deteriorations but the effect on vascular changes has not yet been elucidated. Using multiplexed proteomic and bioinformatic analyses we investigated the molecular mechanisms of vascular pathology in-vitro (primary human umbilical vein endothelial cells, HUVEC) and ex-vivo (arterioles of patients undergoing PD) following exposure to PD-fluid. An overlap of 1813 proteins (40%) of over 3100 proteins was identified in both sample types. PD-fluid treatment significantly altered 378 in endothelial cells and 192 in arterioles. The HUVEC proteome resembles the arteriolar proteome with expected sample specific differences of mainly immune system processes only present in arterioles and extracellular region proteins primarily found in HUVEC. AlaGln-addition to PD-fluid revealed 359 differentially abundant proteins and restored the molecular process landscape altered by PD fluid. This study provides evidence on validity and inherent limitations of studying endothelial pathomechanisms in-vitro compared to vascular ex-vivo findings. AlaGln could reduce PD-associated vasculopathy by reducing endothelial cellular damage, restoring perturbed abundances of pathologically important proteins and enriching protective processes. |
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| DOI: | doi:10.3390/biom10121678 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/biom10121678 |
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| | Volltext: https://www.mdpi.com/2218-273X/10/12/1678 |
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| | DOI: https://doi.org/10.3390/biom10121678 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | span style="font-variant: small-caps"-Alanyl-="font-variant: small-caps"-glutamine |
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| | peritoneal dialysis |
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| | vascular damage |
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| | vasculopathy |
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| K10plus-PPN: | 1788799542 |
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| Verknüpfungen: | → Zeitschrift |
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Peritoneal dialysis fluid supplementation with alanyl-glutamine attenuates conventional dialysis fluid-mediated endothelial cell injury by restoring perturbed cytoprotective responses / Herzog, Rebecca [VerfasserIn]; 15 December 2020 (Online-Ressource)
