| |  Online-Ressource |
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| Verfasst von: | Mahtouk, Karène [VerfasserIn]  |
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| | Hose, Dirk [VerfasserIn] |
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| | Raynaud, Pierre [VerfasserIn] |
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| | Hundemer, Michael [VerfasserIn] |
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| | Jourdan, Michel [VerfasserIn] |
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| | Jourdan, Eric [VerfasserIn] |
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| | Pantesco, Veronique [VerfasserIn] |
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| | Baudard, Marion [VerfasserIn] |
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| | De Vos, John [VerfasserIn] |
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| | Larroque, Marion [VerfasserIn] |
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| | Möhler, Thomas [VerfasserIn] |
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| | Rossi, Jean-Francois [VerfasserIn] |
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| | Rème, Thierry [VerfasserIn] |
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| | Goldschmidt, Hartmut [VerfasserIn] |
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| | Klein, Bernard [VerfasserIn] |
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| Titel: | Heparanase influences expression and shedding of syndecan-1, and its expression by the bone marrow environment is a bad prognostic factor in multiple myeloma |
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| Verf.angabe: | Karène Mahtouk, Dirk Hose, Pierre Raynaud, Michael Hundemer, Michel Jourdan, Eric Jourdan, Veronique Pantesco, Marion Baudard, John De Vos, Marion Larroque, Thomas Moehler, Jean-Francois Rossi, Thierry Rème, Hartmut Goldschmidt, Bernard Klein |
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| E-Jahr: | 2007 |
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| Jahr: | [1 June 2007] |
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| Umfang: | 10 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 11.02.2022 |
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| Titel Quelle: | Enthalten in: Blood |
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| Ort Quelle: | Washington, DC : American Society of Hematology, 1946 |
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| Jahr Quelle: | 2007 |
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| Band/Heft Quelle: | 109(2007), 11, Seite 4914-4923 |
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| ISSN Quelle: | 1528-0020 |
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| Abstract: | The heparan sulfate (HS) proteoglycan, syndecan-1, plays a major role in multiple myeloma (MM) by concentrating heparin-binding growth factors on the surface of MM cells (MMCs). Using Affymetrix microarrays and real-time reverse transcriptase-polymerase chain reaction (RT-PCR), we show that the gene encoding heparanase (HPSE), an enzyme that cleaves HS chains, is expressed by 11 of 19 myeloma cell lines (HMCLs). In HSPE(pos) HMCLs, syndecan-1 gene expression and production of soluble syndecan-1, unlike expression of membrane syndecan-1, were significantly increased. Knockdown of HPSE by siRNA resulted in a decrease of syndecan-1 gene expression and soluble syndecan-1 production without affecting membrane syndecan-1 expression. Thus, HPSE influences expression and shedding of syndecan-1. Contrary to HMCLs, HPSE is expressed in only 4 of 39 primary MMC samples, whereas it is expressed in 36 of 39 bone marrow (BM) microenvironment samples. In the latter, HPSE is expressed at a median level in polymorphonuclear cells and T cells; it is highly expressed in monocytes and osteoclasts. Affymetrix data were validated at the protein level, both on HMCLs and patient samples. We report for the first time that a gene's expression mainly in the BM environment (ie, HSPE) is associated with a shorter event-free survival of patients with newly diagnosed myeloma treated with high-dose chemotherapy and stem cell transplantation. Our study suggests that clinical inhibitors of HPSE could be beneficial for patients with MM. |
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| DOI: | doi:10.1182/blood-2006-08-043232 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1182/blood-2006-08-043232 |
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| | DOI: https://doi.org/10.1182/blood-2006-08-043232 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Aged |
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| | Bone Marrow |
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| | Bone Marrow Cells |
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| | Cell Line, Tumor |
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| | Gene Expression Regulation, Enzymologic |
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| | Gene Expression Regulation, Neoplastic |
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| | Glucuronidase |
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| | Humans |
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| | Middle Aged |
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| | Monocytes |
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| | Multiple Myeloma |
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| | Neutrophils |
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| | Osteoclasts |
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| | Prognosis |
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| | Syndecan-1 |
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| K10plus-PPN: | 1789465524 |
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| Verknüpfungen: | → Zeitschrift |
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Heparanase influences expression and shedding of syndecan-1, and its expression by the bone marrow environment is a bad prognostic factor in multiple myeloma / Mahtouk, Karène [VerfasserIn]; [1 June 2007] (Online-Ressource)
