Transient transfection of plasmodium vivax blood-stage parasites

• Verfasst von: Sanchez, Cecilia P. [VerfasserIn]
• Verfasst von: Pfahler, Judith Martha [VerfasserIn]
• Verfasst von: del Portillo, Hernando A. [VerfasserIn]
• Verfasst von: Lanzer, Michael [VerfasserIn]
• Titel: Transient transfection of plasmodium vivax blood-stage parasites
• Verf.angabe: Cecilia P. Sanchez, Judith Pfahler, Hernando A. del Portillo, Michael Lanzer
• Jahr: 2013
• Umfang: 9 S.
• Fussnoten: First online: 25 August 2012 ; Gesehen am 17.02.2022
• ISBN: 978-1-62703-026-7
• Titel Quelle: Enthalten in: Malaria
• Ausgabe Quelle: 2nd ed. 2013
• Ort Quelle: Totowa, NJ : Humana Press, 2013
• Jahr Quelle: 2013
• Band/Heft Quelle: (2013), Seite 151-159
• DOI: doi:10.1007/978-1-62703-026-7_10
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Ex vivo culture
• Sach-SW: Luciferase assay
• Sach-SW: P. vivax
• Sach-SW: Saimiri monkey
• Sach-SW: Transfection
• K10plus-PPN: 1789934877

Abstract

Here, we describe the methodology for transient transfection of Plasmodium vivax. The ability to genetically manipulate P. vivax has rendered this important human malaria parasite more amenable to molecular investigation. However, a systematic analysis of this parasite and its disease-mediating interactions with the human host still awaits further technological breakthroughs, foremost the establishment of a continuous in vitro culture system. Nevertheless, the first steps towards domesticating P. vivax for research purposes have been made. Transfection will eventually help to better understand the unique pathogenic features displayed by P. vivax, such as the host cell specificity for reticulocytes and the occurrence of relapses. Transfection will also be an invaluable tool for studies related to the emerging drug resistance, and it will help identify and validate novel targets for rational intervention.