Addition of vardenafil into storage solution protects the endothelium in a hypoxia-reoxygenation model

• Verfasst von: Veres, Gábor [VerfasserIn]
• Verfasst von: Hegedűs, Péter [VerfasserIn]
• Verfasst von: Barnucz, Enikő [VerfasserIn]
• Verfasst von: Zöller, Raphael [VerfasserIn]
• Verfasst von: Radovits, Tamás [VerfasserIn]
• Verfasst von: Korkmaz-İçöz, Sevil [VerfasserIn]
• Verfasst von: Kolonics, F. [VerfasserIn]
• Verfasst von: Weymann, Alexander [VerfasserIn]
• Verfasst von: Karck, Matthias [VerfasserIn]
• Verfasst von: Szabó, Gábor [VerfasserIn]
• Titel: Addition of vardenafil into storage solution protects the endothelium in a hypoxia-reoxygenation model
• Verf.angabe: G. Veres, P. Hegedűs, E. Barnucz, R. Zöller, T. Radovits, S. Korkmaz, F. Kolonics, A. Weymann, M. Karck, G. Szabó
• E-Jahr: 2013
• Jahr: 7 June 2013
• Umfang: 7 S.
• Fussnoten: Gesehen am 24.02.2022
• Titel Quelle: Enthalten in: European journal of vascular and endovascular surgery
• Ort Quelle: New York, NY : Elsevier, 1995
• Jahr Quelle: 2013
• Band/Heft Quelle: 46(2013), 2, Seite 242-248
• ISSN Quelle: 1532-2165
• DOI: doi:10.1016/j.ejvs.2013.05.006
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cGMP
• Sach-SW: Endothelium
• Sach-SW: Ischaemia-reperfusion injury
• Sach-SW: Storage
• Sach-SW: Vardenafil
• K10plus-PPN: 1793828814

Abstract

Objective - Based upon the well known protective effect of intracellular cyclic guanosine monophosphate (cGMP) accumulation, we tested the hypothesis that storage solution enriched with optimal concentration of the phosphodiestherase-5 inhibitor vardenafil could provide better protection of vascular grafts against reperfusion injury after long-term cold ischaemic storage. - Methods - Isolated thoracic aorta obtained from rats underwent 24-h cold ischaemic preservation in physiological saline or vardenafil (10−11 M)-supplemented saline solution. Reperfusion injury was simulated by hypochlorite (200 μM) exposure for 30 minutes. Endothelium-dependent vasorelaxation was assessed, and histopathological and molecular-biological examination of the aortic tissue were performed. - Results - Compared with the control group, the saline group showed significantly attenuated endothelium-dependent maximal relaxation (Rmax) to acetylcholine after hypoxia-reoxygenation, which was significantly improved by vardenafil supplementation (Rmax control: 98 ± 1%; saline: 48 ± 6%; vardenafil: 75 ± 4%; p < .05). Vardenafil treatment significantly reduced DNA strand breaks (control: 10.6 ± 6.2%; saline: 72.5 ± 4.0%; vardenafil: 14.2 ± 5.2%; p < .05) and increased cGMP score in the aortic wall (control: 8.2 ± 0.6; saline: 4.5 ± 0.3; vardenafil: 6.7 ± 0.6; p < .05). - Conclusions - Our results support the view that impairment of intracellular cGMP signalling plays a role in the pathogenesis of the endothelial dysfunction induced by cold storage warm reperfusion, which can be effectively reversed by pharmacological phosphodiesterase-5 inhibition.