| |  Online-Ressource |
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| Verfasst von: | Hug, Andreas [VerfasserIn]  |
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| | Storch-Hagenlocher, Brigitte [VerfasserIn] |
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| | Haas, Jürgen [VerfasserIn] |
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| | Vogt-Schaden, Maria-Elisabeth [VerfasserIn] |
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| | Goldschmidt, Hartmut [VerfasserIn] |
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| | Wildemann, Brigitte [VerfasserIn] |
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| Titel: | Single-cell PCR analysis of the immunoglobulin heavy-chain CDR3 region for the diagnosis of leptomeningeal involvement of B-cell malignancies using standard cerebrospinal fluid cytospins |
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| Verf.angabe: | Andreas Hug, Brigitte Storch-Hagenlocher, Juergen Haas, Maria-Elisabeth Vogt-Schaden, Hartmut Goldschmidt, Brigitte Wildemann |
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| E-Jahr: | 2004 |
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| Jahr: | [2004] |
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| Umfang: | 6 S. |
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| Fussnoten: | Gesehen am 25.02.2022 |
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| Titel Quelle: | Enthalten in: Journal of the neurological sciences |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1964 |
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| Jahr Quelle: | 2004 |
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| Band/Heft Quelle: | 219(2004), 1, Seite 83-88 |
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| ISSN Quelle: | 1878-5883 |
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| Abstract: | The diagnosis of leptomeningeal B-cell malignancies is based on the identification of malignant B cells in the cerebrospinal fluid (CSF). We have established a polymerase chain reaction (PCR) approach to characterize the clonally diverse gene encoding the immunoglobulin heavy-chain (IgH) third complementarity determining region (CDR3) of single B cells. We demonstrate that single-cell PCR is readily applicable to individual cells derived from routine CSF cytospins and is a powerful method to discriminate monoclonal neoplastic from polyclonal reactive B-cell responses. Single-cell PCR analysis, as a new tool for the diagnosis and monitoring of neoplastic meningitis associated with B-cell malignancies, is particularly important if cytology, immunocytochemistry, flow cytometry and automated gene scanning of CSF samples are unable to detect malignant monoclonal proliferation. |
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| DOI: | doi:10.1016/j.jns.2003.12.012 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.jns.2003.12.012 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0022510X03003873 |
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| | DOI: https://doi.org/10.1016/j.jns.2003.12.012 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | B-cell leukemia |
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| | B-cell lymphoma |
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| | Cerebrospinal fluid |
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| | Complementarity determining region 3 |
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| | Multiple myeloma |
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| | Neoplastic meningitis |
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| | Single-cell polymerase chain reaction |
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| K10plus-PPN: | 179395108X |
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| Verknüpfungen: | → Zeitschrift |
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Single-cell PCR analysis of the immunoglobulin heavy-chain CDR3 region for the diagnosis of leptomeningeal involvement of B-cell malignancies using standard cerebrospinal fluid cytospins / Hug, Andreas [VerfasserIn]; [2004] (Online-Ressource)
