Expression of HOXC8 is inversely related to the progression and metastasis of pancreatic ductal adenocarcinoma

• Verfasst von: Adwan, Hassan [VerfasserIn]
• Verfasst von: Zhivkova-Galunska, M. [VerfasserIn]
• Verfasst von: Georges, R. [VerfasserIn]
• Verfasst von: Eyol, E. [VerfasserIn]
• Verfasst von: Kleeff, J. [VerfasserIn]
• Verfasst von: Giese, Nathalia [VerfasserIn]
• Verfasst von: Friess, H. [VerfasserIn]
• Verfasst von: Bergmann, Frank [VerfasserIn]
• Verfasst von: Berger, M. R. [VerfasserIn]
• Titel: Expression of HOXC8 is inversely related to the progression and metastasis of pancreatic ductal adenocarcinoma
• Verf.angabe: H. Adwan, M. Zhivkova-Galunska, R. Georges, E. Eyol, J. Kleeff, N.A. Giese, H. Friess, F. Bergmann and M.R. Berger
• E-Jahr: 2011
• Jahr: 28 June 2011
• Umfang: 8 S.
• Fussnoten: Gesehen am 28.02.2022
• Titel Quelle: Enthalten in: British journal of cancer
• Ort Quelle: Edinburgh : Nature Publ. Group, 1999
• Jahr Quelle: 2011
• Band/Heft Quelle: 105(2011), 2, Seite 288-295
• ISSN Quelle: 1532-1827
• DOI: doi:10.1038/bjc.2011.217
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe: Adwan, Hassan, 1972 - : Expression of HOXC8 is inversely related to the progression and metastasis of pancreatic ductal adenocarcinoma. - 2011
• Sach-SW: Cancer genetics
• Sach-SW: Metastasis
• Sach-SW: Pancreatic cancer
• K10plus-PPN: 1794026258

Abstract

Background: The transcription factor HOXC8 regulates many genes involved in tumour progression. This study was to investigate the role of HOXC8 in pancreatic ductal adenocarcinoma (PDAC) growth and metastasis. Methods: The Hoxc8 expression was determined in 15 PDAC cell lines and human specimens by RT–polymerase chain reaction and/or immunohistochemistry. The effects of HOXC8 silencing by RNA interference were investigated by functional tests. Results: The Hoxc8 mRNA expression in PDAC cell lines was negatively related to their growth in vivo. Except for Suit2-007 cells, only those with low Hoxc8 mRNA expression grew in nude rats. Successful down-regulation of HOXC8 expression caused increased proliferation, migration (P⩽0.05) and colony formation (P⩽0.05) in Suit2-007, Panc-1 and MIA PaCa-2 PDAC cells, respectively. The Hoxc8 mRNA levels in diseased human pancreas tissues were significantly increased over normal in PDAC and autoimmune chronic pancreatitis specimens (P<0.01, respectively), but negatively related to tumour stage (P=0.09). In primary and metastatic tumour samples, immunohistochemical staining for HOXC8 was stronger in surrounding than in neoplastic tissues. Furthermore, grading of primary carcinomas was negatively associated with HOXC8 staining (P=0.03). Liver metastases showed the lowest HOXC8 expression of all neoplastic lesions. Conclusion: These data indicate that HOXC8 expression is inversely related to PDAC progression and metastasis.